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聚乙烯二甲 (PET) 微和纳米塑料颗粒影响人类大脑血管细胞的线粒体效率,而不会诱导氧化应激

Sean M Gettings¹, William Timbury¹, Anna Dmochowska²

¹UK Health Security Agency, Radiation Effects Department, Radiation Protection Science Division, Harwell Science Campus, Didcot, Oxfordshire OX11 0RQ, UK.

|April 25, 2024

概括

聚乙烯二甲 (PET) 微/纳米塑料在人类大脑细胞中没有引起氧化应激. 然而,短期PET暴露损害了线粒体功能,这表明潜在的线粒体反应涉及TFAM基因表达.

科学领域:

环境健康科学环境健康科学
毒理学毒理学毒理学
细胞生物学细胞生物学

背景情况:

塑料污染,特别是聚乙烯四甲酸盐 (PET),对环境和健康构成潜在风险.
人类大脑血管周细胞对于血脑屏障的完整性至关重要.
了解微型和纳米塑料对神经细胞的影响至关重要.

研究的目的:

研究PET微/纳米塑料对人类大脑血管周细胞的影响.
评估对线粒体功能和氧化应激的影响.
分析与氧化应激,铁亡和线粒体功能相关的基因表达.

主要方法:

在人脑血管周细胞体外暴露于PET颗粒 (50微克/毫升) 3,6和10天.
对氧化应激标志物的评估.
测量线粒体呼吸 (最大呼吸,备用呼吸能力,ATP生产).
分析线粒体DNA拷贝数和基因表达 (TFAM,氧化应激,铁亡标志物).

主要成果:

在测试度下,PET颗粒不会在皮细胞中诱导氧化应激.
在3天后观察到线粒体呼吸和ATP生产的短暂减少,在6天后恢复.
没有发现线粒体DNA拷贝数或氧化应激/铁灭基因表达的显著变化.
3天后,人们注意到线粒体转录因子A (TFAM) 基因的表达增加.

结论:

PET微/纳米塑料 (50微克/毫升) 不会在人类大脑血管周细胞中诱导氧化应激.
短期PET暴露会损害线粒体功能,这可能表明线粒体质量反应.
基因TFAM可能在观察到的细胞对PET暴露的反应中发挥作用.
需要对线粒激进,血脑屏障完整性和细胞间相互作用进行进一步的研究.

关键词:

微塑料是一种微塑料.线粒体中的线粒体.纳米塑料的纳米塑料氧化应激是一种氧化应激.在这里,PET是PET.细胞 (Pericytes) 是一种细胞.聚乙烯四甲酸是一种聚乙烯四甲酸.

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