皮克林乳液具有高离子强度阻力,由豆蛋白分离物-多糖醇结合颗粒稳定,具有良好的生物相容性
Yijie Wang1, Yunpeng Xie1, Ting Li1
1The Key Laboratory of Synthetic and Biological Colloids, School of Chemical and Material Engineering, Jiangnan University, 1800 Lihu Road, Wuxi 214122, Jiangsu, China.
International journal of biological macromolecules
|April 25, 2024
概括
化学修饰的豆蛋白分离物-多糖醇 (PPI-PG) 结合物增强了皮克林乳液的稳定性. 这些生物相容颗粒提供了更好的pH,热和离子强度抵抗,在食品和化品中具有潜力.
科学领域:
- 生物聚合物修饰的修饰方法
- 合体和接口科学科学
- 材料化学 材料化学
背景情况:
- 蛋白质颗粒是皮克林乳液的关键,但对pH和温度等环境因素敏感.
- 现有的以蛋白质为基础的乳化剂在稳定性和功能方面存在局限性.
研究的目的:
- 开发化学修饰的豆蛋白分离物-多糖醇 (PPI-PG) 结合物,以提高皮克林乳液稳定性.
- 为了研究化学修饰对颗粒性质和乳液性能的影响.
主要方法:
- 豆蛋白分离物 (PPI) 通过希夫基反应与多糖醇 (PG) 进行化学修饰,形成PPI-PG结合物.
- 结合性质的特征,包括分散性,同电点和粒子异质性.
- 在不同的pH值,温度和离子强度条件下评估乳液稳定性.
主要成果:
- PPI-PG合物比纯 PPI 具有 3.5 倍的分散性和增强的生物相容性 (177%的细胞增殖).
- 经过修改的颗粒显示出更好的pH稳定性 (pH3-9),热稳定性 (80°C,48h) 和高离子强度阻力 (3000mM).
- 由此产生的皮克林乳液显示出显著的抗氧化特性,只有10%的降低.
结论:
- PPI与PG的希夫基结合产生了强大的,生物相容的颗粒,用于稳定的皮克林乳液.
- 这些改性颗粒克服了原生蛋白质的局限性,提供了广泛的适用性.
- 潜在的应用范围包括食品,化品和制药行业,因为其稳定性和功能性得到了增强.
相关概念视频
01:07Cohesion
Cohesion is the attraction between molecules of the same type, such as water molecules. Water molecules have an overall neutral charge but are polar molecule. An oxygen atom in one water molecule has a partial negative charge that can bind to a hydrogen atom with a partial positive charge in a second water molecule, forming a hydrogen bond. Each water molecule can form up to four hydrogen bonds with other water molecules. Hydrogen bonds are responsible for water's cohesive nature.
On a surface,...
On a surface,...
01:18Detergent Purification of Membrane Proteins
Detergents are used to purify the integral proteins of the membrane. The hydrophobic portion of the detergent can replace membrane phospholipids while solubilizing the membrane proteins. When detergent monomers reach a specific concentration in a solution called critical micelle concentration (CMC), they form micelles. Above CMC, the concentration of the detergent monomers remains in equilibrium with the micelle. The number of detergent monomers present in the CMC varies for each detergent, and...
00:55Laminins are the Adhesive Proteins of Basal Lamina
Laminins are heterotrimeric proteins with high molecular mass found in the extracellular matrix. Each laminin molecule is composed of three chains, viz. alpha, beta, and gamma, coded by five, four, and three paralogous genes, respectively. Laminins are categories based on the compositions of the three chains.
In humans, the five forms of alpha chains are LAMA 1, LAMA 2, LAMA 3, LAMA 4, and LAMA 5. The four forms of beta chains are LAMB 1, LAMB 2, LAMB 3, and LAMB 4. The three forms of gamma...
In humans, the five forms of alpha chains are LAMA 1, LAMA 2, LAMA 3, LAMA 4, and LAMA 5. The four forms of beta chains are LAMB 1, LAMB 2, LAMB 3, and LAMB 4. The three forms of gamma...
01:18Surface Membrane Barriers
The skin and mucous membranes serve as the primary line of defense against pathogens by providing both physical and chemical protection. These barriers are essential in preventing the entry and establishment of microbes, thereby maintaining the integrity of the host.
The outer layer of the skin, the epidermis, is a robust barrier comprising layers of closely packed keratinized cells. This dense arrangement prevents microbes from penetrating the body. The periodic shedding of epidermal cells...
The outer layer of the skin, the epidermis, is a robust barrier comprising layers of closely packed keratinized cells. This dense arrangement prevents microbes from penetrating the body. The periodic shedding of epidermal cells...
01:30Micelles
Micelle formation is an intricate process that hinges on the properties of amphiphilic or amphipathic molecules and the conditions of the system in which they are found. Amphiphilic molecules, which have both hydrophilic (water-attracting) and hydrophobic (water-repelling) parts, play a critical role in this process.In aqueous environments, these molecules arrange themselves such that their hydrophilic heads are turned towards the water phase, while their hydrophobic tails are oriented away...
01:29Biofilms
Biofilms are complex communities of microorganisms encased in a self-produced extracellular polysaccharide matrix attached to surfaces. These microbial consortia can include single or multiple species, providing enhanced survival benefits by forming organized, multilayered structures.The formation of biofilms occurs through four key stages: attachment, colonization, development, and dispersal.During attachment, free-swimming planktonic cells adhere to a surface, often facilitated by...