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表皮-介质细胞转化通过NF-κB/MMP9轴促进肝细胞癌的进展
Jinsen Li1, Qingbin Cai2, Ziyue Wang3
1Nuclear Magnetic Resonance Department, Third Affiliated Hospital of Qiqihar Medical College, 161000 Qiqihar, Heilongjiang, China.
Discovery medicine
|April 26, 2024
概括
通过导管动脉化学栓塞 (TACE) 在肝癌中的有效性与上皮-介质细胞过渡 (EMT) 有关. 抑制NF-κB和MMP9通过调节肝细胞癌细胞中的EMT来减少扩散并阻碍细胞亡.
科学领域:
- 在瘤学瘤学.
- 分子生物学分子生物学
- 肝病学 肝病学是一种肝病学.
背景情况:
- 初级肝癌 (PHC) 是一种普遍存在的恶性瘤.
- 通过导管动脉化学栓塞 (TACE) 改善了PHC患者的治疗结果.
- 在肝细胞癌 (HCC) 的TACE疗效中,上皮细胞-介质细胞过渡 (EMT) 的作用需要阐明.
研究的目的:
- 调查EMT对HCC的影响.
- 探索EMT与TACE治疗结果相关的潜在机制.
主要方法:
- 对接受TACE的晚期肝癌患者的回顾性分析,按临床疗效分类.
- 基因表达的评估 (NF-κB,MMP9,Ki-67,Bcl-2,Bax,Vimentin,E-cadherin,N-cadherin) 通过RT-PCR和西方模拟.
- 在实验室中使用Huh7细胞进行的临床研究,该研究中使用了透视病毒介导的NF-κB和MMP9的抑制,评估了EMT和细胞活力.
主要成果:
- 在无效TACE组中观察到NF-κB,MMP9,Ki-67,Bcl-2,Vimentin和N-cadherin的表达更高,Bax和E-cadherin的表达更低.
- 在体外,对NF-κB和MMP9的抑制显著降低了增殖标志物 (Ki-67,Bcl-2,Vimentin,N-cadherin) 和增加了亡标志物 (Bax,E-cadherin).
- 抑制NF-κB和MMP9导致Huh7细胞的细胞活力增加和细胞亡减少.
结论:
- NF-κB/MMP9信号通路是HCC中扩散和亡的关键调节者.
- 通过NF-κB/MMP9轴对EMT的调节会影响肝细胞癌细胞的行为.
- 准NF-κB/MMP9通路可能为改善HCCTACE疗效提供治疗策略.
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