通过敏感的HILIC-MS/MS方法对压力生物标志物的生物分析:迈向MDA,ACR和CTA准确量化的一步
Harsh Thakkar1, Shriya Gangakhedkar1, Ravi P Shah1
1Department of Pharmaceutical Analysis, National Institute of Pharmaceutical Education and Research-Ahmedabad (NIPER-A), Gandhinagar 382355, India.
Journal of the American Society for Mass Spectrometry
|April 26, 2024
概括
现在可以准确量化反应性化物,主要的氧化应激生物标志物. 使用histidine衍生物化的新HILIC-MS/MS方法为生物样本提供了高特异性和可重复性.
科学领域:
- 生物化学 生物化学
- 分析化学 分析化学
- 生物标志物发现发现
背景情况:
- 像马隆迪甲基这样的反应性化物是氧化应激的关键生物标志物.
- 现有的分析方法往往缺乏特异性,导致由于恶劣条件和假阳性结果导致不准确的量化.
研究的目的:
- 开发一种高特异性和可重复的方法来量化生物矩阵中的反应性化物.
- 解决当前氧化应激评估分析技术的局限性.
主要方法:
- 开发了一种新的水友互动液体染色学-并联质谱法 (HILIC-MS/MS) 方法.
- 内源性歇斯蒂丁被用于衍生,以克服阿尔代的反应性.
- 使用核磁共振 (NMR) 和液体染色学-高分辨率质谱 (LC-HRMS) 进行了衍生 adduct 的表征.
- 为了准确量化,使用多重反应监测 (MRM).
主要成果:
- 开发的HILIC-MS/MS方法证明了用于量化马隆迪化,阿克罗莱因和克罗托纳尔化的增强特异性和可重复性.
- 希斯蒂丁衍生物有效地稳定了生物样本中的反应性化物.
- 坐标性表征证实了衍生引证的结构.
结论:
- 这种新方法为准确量化反应性化物提供了明确的解决方案.
- 该技术适用于在体内和体外研究中评估氧化应激.
- 提高生物标志物量化准确度有助于更好地了解与氧化压力相关的条件.
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