怀孕会影响干扰素刺激基因的表达,孕激素受体和孕激素诱导的阻断因子在绵羊甲状腺
Jianhua Cao1, Shuxin Zhao1, Yaqi Zhang1
1School of Life Sciences and Food Engineering, Hebei University of Engineering, Handan 056038, China.
Animal bioscience
|April 26, 2024
概括
早期怀孕通过改变干扰素刺激基因 (ISG) 和激素受体表达来影响绵羊甲状腺功能. 这些由胚胎干扰素-tau (IFNT) 和孕激素驱动的变化可能会影响甲状腺自身免疫和激素分泌.
科学领域:
- 生殖免疫学 生殖免疫学
- 内分泌学 在内分泌学.
- 绵羊的生理学 绵羊的生理学
背景情况:
- 胚胎干扰素tau (IFNT) 和孕激素对于确定怀孕至关重要.
- 甲状腺功能可以受到生殖荷尔蒙和免疫因素的影响.
- 干扰素刺激基因 (ISG),孕激素受体 (PGR) 和孕激素诱导阻断因子 (PIBF) 是关键的分子参与者.
研究的目的:
- 调查胚胎IFNT和孕激素对ISG,PGR和PIBF在怀孕早期羊甲状腺表达的影响.
- 了解母亲甲状腺中这些分子标记物的时间变化.
主要方法:
- 甲状腺组织从不同阶段的母羊中收集:非怀孕的第16天,怀孕的第13,16和25天.
- 实时定量聚合酶连锁反应 (qPCR),西部污染和免疫组织化学被使用.
- 分析了ISG (包括ISG15,IP-10,MX1),PGR异型,PIBF和STAT1的表达水平.
主要成果:
- 检测到ISG15蛋白质的结合形式,ISG15结合物,PGR (70kDa),PIBF,IP-10和MX1在怀孕16天的峰值表达.
- 在怀孕16日,STAT1表达最低.
- 在怀孕的第25天,PGR (70kDa) 和STAT1水平下降,而PGR (43kDa),2',5'-奥利戈亚丁酸合成酶,IP-10和MX1水平与雌性周期相比增加.
结论:
- 早期怀孕显著改变了ISGs,PGR和PIBF在羊的甲状腺中的表达.
- 胚胎IFNT和孕激素可能是这些甲状腺变化的媒介.
- 这些荷尔蒙和免疫调节可能在调节甲状腺自身免疫和荷尔蒙分泌过程中发挥作用在怀孕早期在羊.
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