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在物种中广泛使用酸 D人类腺病毒

Rosie M Mundy¹, Alexander T Baker¹, Emily A Bates¹

¹Division of Cancer & Genetics, Cardiff University School of Medicine, Cardiff, CF14 4XN UK.

|April 26, 2024

概括

人类腺病毒 (HAdV) D 种,经常引起眼睛感染,利用酸 (SA) 进入细胞. 了解这些相互作用有助于开发新的抗病毒疗法和病毒载体.

科学领域:

病毒学病毒学
结构生物学结构生物学
传染性疾病传染性疾病

背景情况:

人类腺病毒 (HAdV) 是常见的病原体,其中D种 (HAdV-D) 与胃肠道感染和流行性角质结膜炎 (EKC) 有关.
进入HAdV-D细胞的确切机制尚不清楚.
酸 (SA) 被认为是导致EKC的HAdV-D菌株的潜在细胞受体.

研究的目的:

调查酸 (SA) 在人类腺病毒物种D (HAdV-D) 细胞感染机制中的作用.
阐明HAdV-D纤维旋蛋白与SA.的相互作用的结构基础.
评估SA和Coxsackie和腺病毒受体 (CAR) 对HAdV-D感染性的贡献.

主要方法:

确定了七种HAdV-D纤维旋蛋白的Apo状态晶体结构.
获得了HAdV-D25,D29,D30和D53纤维旋蛋白与酸 (SA) 复合体中的晶体结构.
在细胞表面SA去除后显示HAdV-D纤维旋的HAdV-C5载体的评估感染性和评估CAR参与.

主要成果:

为七个以前未被描述的HAdV-D纤维旋蛋白提供了高分辨率的晶体结构.
揭示了HAdV-D25,D29,D30和D53纤维旋与SA的结构复合体,详细介绍了结合相互作用.
证明减少细胞表面SA显著降低了具有HAdV-D纤维旋伪型的HAdV-C5载体的传染性.
观察到对SA和Coxsackie和腺病毒受体 (CAR) 对HAdV-D感染性的可变依赖.

结论:

许多HAdV-D菌株使用酸 (SA) 作为它们进入细胞通路的关键因素.
结构数据为了解SA介导的HAdV-D热流提供了基础.
SA和CAR的可变使用突出了HAdV-D中的多样性,对于开发向抗病毒药物和工程病毒载体至关重要.

关键词:

亚地诺病毒是亚地诺病毒.病毒载体病毒载体病毒结构病毒结构

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