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Updated: Jun 27, 2025

Longitudinal Morphological and Physiological Monitoring of Three-dimensional Tumor Spheroids Using Optical Coherence Tomography
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Longitudinal Morphological and Physiological Monitoring of Three-dimensional Tumor Spheroids Using Optical Coherence Tomography

Published on: February 9, 2019

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光热光学连贯性断层扫描用于3D活细胞检测和映射

Jingyu Sun1, Tianqi Fang1, Hongjun Wang1

  • 1Department of Biomedical Engineering, Stevens Institute of Technology, Hoboken, NJ 07030, USA.

Optics continuum
|April 26, 2024
PubMed
概括

光热光学一致性断层扫描 (PT-OCT) 能够在散射介质中对活的纳米粒子标记细胞进行3D成像. 这种技术可以在没有细胞死亡的情况下检测和绘制单细胞,补充现有的显微镜方法.

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Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.
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