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相关概念视频

Fates of Pyruvate01:20

Fates of Pyruvate

8.4K
Pyruvate is the end product of glycolysis, where glucose is oxidized to pyruvate, simultaneously reducing NAD+ to NADH. Two molecules of ATP are also produced by substrate-level phosphorylation.
In aerobic organisms, pyruvate is metabolized via the citric acid cycle to produce reduced coenzymes NADH and FADH2. These coenzymes are then oxidized in the electron transport chain to produce ATP and, in the process, regenerate the NAD+ and FAD. As seen in some cell types and organisms, fermentation...
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Fermentation01:29

Fermentation

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Most eukaryotic organisms require oxygen to survive and function adequately. Such organisms produce large amounts of energy during aerobic respiration by metabolizing glucose and oxygen into carbon dioxide and water. However, most eukaryotes can generate some energy in the absence of oxygen by anaerobic metabolism.
Fermentation is a type of metabolic process that occurs in the absence of oxygen, where organic molecules such as glucose are broken down to produce energy. During this process, the...
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相关实验视频

Updated: Jun 27, 2025

Medium Preparation for the Cultivation of Microorganisms under Strictly Anaerobic/Anoxic Conditions
06:17

Medium Preparation for the Cultivation of Microorganisms under Strictly Anaerobic/Anoxic Conditions

Published on: August 15, 2019

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两个阶段的微发酵.

Shuai Li1, Zhixia Ye2, Eirik A Moreb2

  • 1Department of Chemistry, Duke University, Durham, NC, USA.

Metabolic engineering communications
|April 26, 2024
PubMed
概括
此摘要是机器生成的。

工程细胞工厂提供了多样化的产品可能性. 新的协议标准化了使用两阶段发酵过程评估这些微生物生产菌株,简化了菌株工程和扩展.

关键词:
第二阶段:第二阶段.动态控制 动态控制 动态控制美国大肠杆菌 (E. coli).高吞吐率的高吞吐量.代谢工程是代谢工程.微生物菌株评估评估微发酵是一种微发酵.酸盐是一种酸盐.蛋白质表达方式 蛋白质表达方式

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Techniques for the Evolution of Robust Pentose-fermenting Yeast for Bioconversion of Lignocellulose to Ethanol

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相关实验视频

Last Updated: Jun 27, 2025

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06:17

Medium Preparation for the Cultivation of Microorganisms under Strictly Anaerobic/Anoxic Conditions

Published on: August 15, 2019

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Light-Controlled Fermentations for Microbial Chemical and Protein Production
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Light-Controlled Fermentations for Microbial Chemical and Protein Production

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Techniques for the Evolution of Robust Pentose-fermenting Yeast for Bioconversion of Lignocellulose to Ethanol
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科学领域:

  • 合成生物学 合成生物学
  • 代谢工程是代谢工程.
  • 生物技术是生物技术.

背景情况:

  • 在DNA合成和基因组编辑方面的进步使得大量工程细胞工厂变体的快速生成成为可能.
  • 增加菌株变异生成需要标准化,高通量评估方法.
  • 之前的工作建立了工程 *E. coli* 菌株和一个分离生长和生产的 2 阶段生产过程.

研究的目的:

  • 详细阐述了在2阶段微发酵中评估工程*大肠杆菌*菌株的协议的开发.
  • 为评估细胞工厂性能提供标准化的方法.
  • 为各种产品,主机和触发器适应这些协议.

主要方法:

  • 开发用于二阶段微发酵的详细方案.
  • 利用酸盐枯竭作为一个触发器来停止生长并诱导异质表达.
  • 专注于将细胞生长 (第一阶段) 与静止阶段 (第二阶段) 的产品形成脱.

主要成果:

  • 建立了强大的协议,用于评估微发酵中的工程 * E. coli * 菌株.
  • 为各种蛋白质和小分子产品证明了2阶段工艺的适应性.
  • 为适应不同细胞宿主和触发机制的方法提供了一个框架.

结论:

  • 开发的微发酵协议标准化和简化了工程细胞工厂的评估.
  • 这种方法促进了对菌株变异的高通量评估.
  • 该方法具有适应性,支持合成生物学和生物生产中的更广泛应用.