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使用真菌分离剂K20从发酵的油棕空果束中生产酸:自由细胞和固定细胞之间的比较

Antika Boondaeng¹, Jureeporn Keabpimai¹, Chanaporn Trakunjae¹

¹Kasetsart Agricultural and Agro-Industrial Product Improvement Institute, Kasetsart University, Bangkok, Thailand.

|April 26, 2024

概括

这项研究探讨了使用棕油的空果束来生产酸 (FA),一种食品添加剂. 用真菌分离物K20发酵实现了显著的FA产量,证明了可再生原料应用的潜力.

科学领域:

生物技术是生物技术.
工业微生物学工业微生物学
可再生能源可再生能源是可再生能源.

背景情况:

酸 (FA) 是一种重要的食品添加剂,用于保存和增味.
越来越多地寻求可再生原料来取代化学生产中的传统资源.
棕油的空果束 (EFB) 是一个丰富的纤维素生物质资源.

研究的目的:

为了评估蒸汽爆炸油棕EFB作为酸 (FA) 生产的基质.
为了比较使用自由和固定真菌细胞的FA生产效率.
评估FA发酵过程的可扩展性.

主要方法:

蒸汽爆炸预处理棕油 EFB 提取葡萄糖.
用真菌分离物K20进行发酵,用于FA生产.
自由细胞和固定细胞发酵的比较.
在3L空气升降发酵器中进行了扩大规模的研究.

主要成果:

使用自由细胞的最大FA度为3.25g/L,生产率为0.034g/L/h.
扩大规模的发酵从100g/L的EFB衍生葡萄糖中产生了44g/L的FA.
在发酵器中,获得了0.39g/g的FA产量和0.41g/L/h的生产率.

结论:

蒸汽爆炸油棕EFB是一种可行的可再生原料,用于酸生产.
菌分离物K20从EFB衍生的葡萄糖中表现出有效的FA生物合成.
成功扩大规模表明,该生物工艺的工业应用具有重大潜力.

关键词:

空的水果束是没有的.酸生产酸生产菌分离物K20 菌分离物K20 动不动的细胞被固定起来.棕油是一种油.

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