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在细胞学样本上对双 p16/Ki-67 免疫染的评估

Marie C Smithgall¹, William S Towne¹, Abel A Gonzalez¹

¹Department of Pathology and Cell Biology, Columbia University Irving Medical Center, New York, New York, USA.

Diagnostic cytopathology

|April 26, 2024

概括

双 p16/Ki-67 染色显示出内细胞学的前景,有效地识别高度状细胞内皮病变 (HSIL/AIN2-3),并有助于在不确定的意义非典型状细胞 (ASCUS) 病例的风险分层.

科学领域:

妇科病理学妇科病理学
细胞病理学细胞病理学
在瘤学瘤学.

背景情况:

双 p16 / Ki-67 免疫染被FDA批准用于宫细胞学.
它在细胞学中的实用性,特别是对于ASCUS风险分层,需要进一步研究.

研究的目的:

在细胞学样本中评估双 p16/Ki-67 染色的性能.
为了将染色结果与随后的细胞学解释,高风险HPV状态和脏活检结果相关联.

主要方法:

在43个基于液体的门细胞学标本上进行了双 p16/Ki-67 染色.
结果与细胞学和活检数据一起分析,包括高风险HPV检测.

主要成果:

在活检中,所有三例高度状内皮损伤 (HSIL/AIN2-3) 均为双色染色呈阳性.
所有高风险HPV阴性病例均对双染色呈阴性.
双 p16 / Ki-67 染色显示高特异性 (1.0) 和负预测值 (0.9) 对于任何等级的SIL在活检,超过HR-HPV测试的特异性 (0.44).

结论:

双 p16/Ki-67 染色可以在门细胞学中表明HPV 感染的转变.
它作为一种有价值的辅助测试,用于非典型细胞学的风险分层,特别是ASCUS.
该测试显示了SIL的高特异性和NPV,使其在资源有限的环境中变得有用.

关键词:

这是一个HPVHPVHPV.在 Ki6767 的位置上.门细胞学门细胞学 p16 p16 在这个问题上.状内皮层病变的发生.

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