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O-GlycoIsoQuant:一种通过超基释放和同位素吉拉德的P标签的新型O-Glycome定量方法

Yueyue Li¹, Bin Fu², Yang Li¹

¹Liver Cancer Institute, Zhongshan Hospital and Institutes of Biomedical Sciences, Fudan University, Shanghai 200032, China.

Analytical chemistry

|April 26, 2024

概括

一种名为O-GlycoIsoQuant的新方法,可以精确量化O-glycans,这对于了解癌症等疾病至关重要. 这种技术克服了在生物样本中分析这些复杂的糖结构的挑战.

科学领域:

生物化学生化学
分析化学分析化学
格莱科米克斯 (Glycomics) 是一个有趣的药物.

背景情况:

定量糖化分析对于疾病生物标志物的发现至关重要.
由于稀少和复杂的释放,O-甘氨酸很难通过质谱学进行定量分析.
现有的O-甘氨酸分析方法缺乏效率和通用性.

研究的目的:

开发一种新的,高效的,可重复的定量O-糖分析方法.
为了克服当前O-甘氨酸质谱技术的局限性.
为了能够在复杂的生物样本中敏感检测和相对量化O-甘氨酸.

主要方法:

开发了O-GlycoIsoQuant,一种结合超基辅助释放和同位素标记的方法.
使用1,8-diazabicyclo[5.4.0]undec-7-ene (DBU) 进行快速的非还原性β-消除O-糖.
使用轻和重同位素吉拉德试剂P (GP) 进行基于质谱的相对量化.

主要成果:

O-GlycoIsoQuant表现出高的标签效率和出色的可重复性 (CV < 20%).
该方法在100倍的动态范围中显示出良好的线性 (R2 > 0.99).
成功地应用于各种样本 (血清,唾液,精子,EVs),检测到众多O-glycans.

结论:

O-GlycoIsoQuant为定量O-glycomics提供了一个强大的和广泛适用的解决方案.
这种方法有助于更深入地了解与疾病相关的糖化变化.
能够在各种生物环境中进行敏感和准确的O-甘氨酸分析.

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