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从橄叶中提取和净化橄蛋白

Jalal Hassan¹, Mohammad Kazem Koohi¹, Aghil Sharifzadeh²

¹Division of Toxicology, Department of Comparative Bioscience, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.

Natural product research

|April 26, 2024

概括

乙醇,乙二和水有效提取化合物,橄叶的产量高于水果. 欧蛋白的提取效率取决于溶剂体积,温度和时间.

科学领域:

植物化学植物化学
分析化学分析化学
自然产品提取提取自然产品

背景情况:

化合物具有显著的抗氧化特性.
橄树叶和果实是丰富的生物活性化合物的来源,包括oleuropein.
有效的提取方法对于分离和量化这些有价值的化合物至关重要.

研究的目的:

为了研究不同溶剂 (乙醇,乙二,水) 对于化合物提取的有效性.
为了确定提取参数 (溶剂体积,温度,持续时间) 对产量和抗氧化活性的影响.
为了比较橄叶和果实提取物中的油脂蛋白度,并净化来自叶子的油脂蛋白.

主要方法:

使用乙醇,乙二和水进行溶剂提取.
提取参数的优化:溶剂体积,温度和持续时间.
化合物和抗氧化活性的量化.
液体-液体提取用于欧蛋白净化.

主要成果:

提取效率与溶剂体积,温度和持续时间正相关.
与水果提取物 (1800 mg/L) 相比,橄叶提取物含有较高度的化合物,特别是油脂蛋白 (4900 mg/L).
从橄叶中提取的纯化欧蛋白达到>98%的纯度,在干燥的叶子中度为9.8%w/w.

结论:

溶剂选择和提取条件显著影响化合物产量和抗氧化活性.
与橄果相比,橄叶是欧蛋白的优质来源.
高纯度的欧蛋白可以有效地从橄叶中提取和净化.

关键词:

橄树叶的叶子抗氧化剂活性抗氧化剂活性生物醇是一种生物醇.提取提取提取提取净化净化净化净化

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