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对比子对具有多个永久电荷的分析物的软电离质谱的影响
Olga Kočková1, Petr Kasal2, Jan Zelený2
1Department of Analytical Chemistry, Institute of Macromolecular Chemistry of the Czech Academy of Sciences, Heyrovského nám. 2, Prague 6 162 00, Czech Republic.
Analytical chemistry
|April 26, 2024
概括
其他因素对多重永久充电分析剂 (MPCAs) 的质谱产生显著影响. 最佳的结合强度,受 counterion 基本性和分析物电荷的影响,对于高质量的质谱 (MS) 数据至关重要.
科学领域:
- 分析化学 分析化学
- 质谱测量质量谱测量
- 超分子化学 超分子化学
背景情况:
- 多重永久充电分析剂 (MPCAs) 在各种科学应用中至关重要.
- 质谱法 (MS) 是一个强大的工具来表征MPCAs,但它的有效性受到 counterions的影响.
- 了解 counterions 的作用是通过 MS 优化 MPCA 分析的关键.
研究的目的:
- 为了研究各种单价 counterions 在基于β-cyclodextrin 的 MPCAs 的软电离质谱 (MS) 上的影响.
- 确定最佳的对比特征,以获得MPCA的高质量的MS数据.
- 建立一个解释和改进MPCA质谱的框架.
主要方法:
- 制备基于β-环氧的MPCA,具有不同的电荷 (7和14个四级基).
- 使用两种软电离技术分析MPCA:电喷电离 (ESI) 和矩阵辅助激光脱吸电离 (MALDI).
- 系统变化单价 counterions 评估它们对MS信号质量和分析物分配的影响.
主要成果:
- 马尔迪MS提供了高分辨率的光谱,分析剂信号只观察到弱基本对比子 (ClO4−和TfO−).
- MIM14BCD的ESI MS显示了含有ClO4−和TfO−的分析物信号,而MIM7NBCD的ESI MS显示了含有Cl−但不含有TfO−的信号.
- 一个组合模型表明,根据分析物的电荷,MPCA电荷和对电荷之间的最佳结合强度对于MS质量至关重要.
结论:
- 使用ESI和MALDI MS.成功的MPCA表征,选择对象是至关重要的.
- 使用多余的酸有效地在现场进行对替代,为改善MS数据质量提供了一种实际的解决方案.
- 这项研究为解释和增强MPCA质谱提供了全面的理解.
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