通过元分析来定义型结肠炎中差异表达的lncRNAs的参考集的挑战
Christopher G Fenton1,2, Mithlesh Kumar Ray1, Ruth H Paulssen1,2
1Clinical Bioinformatics Research Group, Department of Clinical Medicine, UiT-The Arctic University of Norway, N-9037 Tromsø, Norway.
Current issues in molecular biology
|April 26, 2024
概括
研究人员使用基因表达数据在性结肠炎 (UC) 中确定了19种常见的差异表达长非编码RNA (lncRNAs). 这些lncRNAs可以作为活跃UC的生物标志物,并与结直肠癌的进展有关.
科学领域:
- 胃肠病学 胃肠病学
- 分子生物学分子生物学
- 生物信息学是一种生物信息学.
背景情况:
- 性结肠炎 (UC) 是一种慢性炎症性肠病.
- 确定活跃UC的可靠生物标志物对于患者管理至关重要.
- 长非编码RNAs (lncRNAs) 在各种生物过程和疾病中发挥作用.
研究的目的:
- 使用公开可用的基因表达数据集,识别性结肠炎 (UC) 中常见的差异表达的lncRNAs.
- 探索这些lncRNAs作为活跃UC生物标志物的潜力.
- 研究已识别的lncRNAs与结直肠癌 (CRC) 途径的关联.
主要方法:
- 从Gene Expression Omnibus (GEO) 的九个人类结肠活检转录数据集的手动策划.
- 使用R包 (geneknitR,gprofiler,clusterProfiler) 来进行基因符号注释.
- 使用R EdgeR包进行差异表达分析,以识别至少三组数据中存在的lncRNA.
主要成果:
- 在多个UC GEO数据集中识别了19个差异表达的lncRNAs.
- 一些已识别的lncRNA与结直肠癌 (CRC) 的进展有关,包括调节基因表达,表皮细胞转化为介质细胞转化 (EMT),细胞周期,增殖,入侵和迁移.
- 根据疾病状态和UC内的组织位置观察到lncRNA表达的变化.
结论:
- 已识别的19个lncRNAs可以作为活跃性结肠炎的一般生物标志物,独立于活检位置,年龄,性别或治疗.
- 这些发现为未来的研究和使用现有的GEO UC数据集进行比较提供了宝贵的资源.
- 鉴定到的lncRNA与CRC通路的关联需要进一步研究它们在炎症和癌症中的双重作用.
相关概念视频
lncRNA - Long Non-coding RNAs
8.6K
In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...
8.6K
Inflammatory Bowel Disease I: Ulcerative Colitis
172
Introduction
Inflammatory bowel disease, or IBD, encompasses a group of disorders characterized by chronic inflammation or ulceration of the gastrointestinal tract.
Risk Factors
The exact cause of IBD remains unclear, although it is believed to be due to a mix of genetic, environmental, microbial, and immune factors. Genetic factors are significant in determining susceptibility to IBD, with family history being a critical risk factor. Individuals with a first-degree relative who has IBD are at...
Inflammatory bowel disease, or IBD, encompasses a group of disorders characterized by chronic inflammation or ulceration of the gastrointestinal tract.
Risk Factors
The exact cause of IBD remains unclear, although it is believed to be due to a mix of genetic, environmental, microbial, and immune factors. Genetic factors are significant in determining susceptibility to IBD, with family history being a critical risk factor. Individuals with a first-degree relative who has IBD are at...
172
Inflammatory Bowel Disease III: Diagnostic Studies and Management I-Nutritional Therapy
331
Various diagnostic tests are employed in the diagnostic process for Inflammatory Bowel Disease (IBD), particularly to differentiate between Crohn's disease and ulcerative colitis.
Diagnostic studies
A colonoscopy is the definitive screening test, distinguishing ulcerative colitis from other colon diseases with similar symptoms. During a colonoscopy test, inflamed mucosa with exudate ulcerations can be observed, and biopsies are taken to determine the histologic characteristics of the...
Diagnostic studies
A colonoscopy is the definitive screening test, distinguishing ulcerative colitis from other colon diseases with similar symptoms. During a colonoscopy test, inflamed mucosa with exudate ulcerations can be observed, and biopsies are taken to determine the histologic characteristics of the...
331
Drugs for Treatment of Ulcerative Colitis in IBD
138
Ulcerative colitis is a chronic inflammatory condition primarily affecting the colon and rectum. The primary drugs used in the treatment of ulcerative colitis are aminosalicylates. They exhibit anti-inflammatory and immunosuppressive properties. They modulate inflammatory mediators and inhibit the activity of nuclear factor κB (NF-κB). Aminosalicylates also reduce inflammation by inhibiting prostaglandin and leukotriene production and decreasing neutrophil chemotaxis and superoxide...
138


