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在正常和恶性人体组织中,RNA表达和雌激素受体基因DNA甲基化之间的相关性

Ju Rong¹, Xiaojun Xie², Yongdong Niu³

¹The First Clinical Institute, Shantou University Medical College, Shantou 515041, China.

Current issues in molecular biology

|April 26, 2024

概括

雌激素受体ERα,ERβ和GPER1在人类组织和癌症中表现出独特的表达模式. 在恶性瘤中,GPER1持续下降,这表明瘤抑制作用,而DNA甲基化影响ERα和GPER1表达和瘤存活.

科学领域:

内分泌学和分子生物学
癌症研究癌症研究
基因组学和表观遗传学

背景情况:

雌激素受体 (ERα,ERβ,GPER1) 对雌激素的功能至关重要.
雌激素受体信号通路已得到充分研究,但它们的组织特异性表达和调节仍然不清楚.
了解这些受体的表达和甲基化对于探索它们在健康和疾病中的作用至关重要.

研究的目的:

综合分析正常和恶性人体组织中雌激素受体 (ERα,ERβ,GPER1) 的表达特征.
研究雌激素受体基因的DNA甲基化状态及其与基因表达和瘤存活的相关性.
发现雌激素信号传递的新生理学和病理生理学功能.

主要方法:

利用人类蛋白质地图,TCGA和GTEx数据库通过GEPIA进行基因表达分析.
使用Wanderer和cBioPortal软件分析了DNA甲基化状态.
使用MethSurv工具评估了CG甲基化和瘤存活率之间的关联.

主要成果:

ERα,ERβ和GPER1在各种组织和癌症中表现出不同的表达模式.
男性丸的ERβRNA表达率高于女性丸; ERαRNA水平与正常组织中的雄激素受体呈正相关.
在多种恶性瘤中,GPER1表达始终下降,表明瘤抑制功能. DNA甲基化与ERα和GPER1表达和瘤存活相关,但并不总是与基因表达水平相关.

结论:

雌激素受体具有独特的表达特征和调节机制.
在癌症中GPER1的表达减少表明潜在的瘤抑制作用.
雌激素受体基因的DNA甲基化影响瘤进展和潜在的治疗反应,独立于直接的基因表达调节.

关键词:

通过DNA甲基化.这种癌症是癌症癌症.雌激素受体的受体是雌激素受体.表达方式表达方式表达方式表达方式表达方式表达方式表达方式表达方式表达方式表达方式表达方式表达方式

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