相关实验视频

Updated: Jun 27, 2025

Characterization of Multi-subunit Protein Complexes of Human MxA Using Non-denaturing Polyacrylamide Gel-electrophoresis

Characterization of Multi-subunit Protein Complexes of Human MxA Using Non-denaturing Polyacrylamide Gel-electrophoresis

Published on: October 28, 2016

对抗病毒跨膜蛋白的进一步表征 MARCH8

Takuya Tada^1,2, Yanzhao Zhang^1,3,4, Dechuan Kong^1,5

¹Department of Pathology, National Institute of Infectious Diseases, Tokyo 162-8640, Japan.

|April 26, 2024

概括

跨膜蛋白MARCH8通过减少细胞表面上的病毒糖蛋白来抑制病毒感染. 它的抗病毒功能在所有物种中都保持不变,具有对活动至关重要的特定域,尽管它不能阻止细胞对细胞的传播.

科学领域:

病毒学病毒学
细胞生物学细胞生物学
免疫学免疫学免疫学

背景情况:

MARCH8是一种已知的细胞跨膜蛋白,可降低病毒包膜糖蛋白的调节.
这种由MARCH8降低的调节,通过限制糖蛋白合并到病毒中来降低病毒感染的效率.

研究的目的:

为了描述MARCH8抗病毒活性的物种特异性.
确定MARCH8中负责其抗病毒功能的特定域.
评估MARCH8在抑制细胞间HIV-1感染方面的有效性.

主要方法:

使用 rhesus macaque,小鼠和牛版本,研究了MARCH8的物种特异性.
分析了RING-CH域和氨酸基因的功能重要性.
在MARCH8和MARCH3之间构建了嵌合蛋白来映射关键域.
评估了HIV-1 Env和VSV-G伪病毒感染的抑制.
评估了细胞对细胞HIV-1感染的抑制.

主要成果:

MARCH8的抗病毒功能在 rhesus ,小鼠和牛种类中保持着.
环CH域对于抑制HIV-1 Env和VSV-G伪病毒感染至关重要.
铁素基因对于抑制HIV-1 Env至关重要,但不是VSV-G.
无论是N端和C端的细胞质尾巴,以及N端的跨膜域,都对抗病毒活性至关重要.
由于没有足够的Env降低调节,MARCH8不能抑制细胞间HIV-1感染.

结论:

MARCH8在多种物种中表现出保存的抗病毒特性.
包括RING-CH和细胞质尾巴在内的特定域对MARCH8的抗病毒机制至关重要.
三月八号无法阻止细胞间传播,这凸显了它对抗HIV-1的广泛抗病毒能力的局限性.

关键词:

艾滋病病毒艾滋病病毒艾滋病病毒 3月8日时间:这是一个VSV-G.抗病毒活动的抗病毒活性.细胞对细胞的感染.种类的特异性种类的特异性

更多相关视频

Engineering Antiviral Agents via Surface Plasmon Resonance

Engineering Antiviral Agents via Surface Plasmon Resonance

Published on: June 14, 2022

Use of Viral Entry Assays and Molecular Docking Analysis for the Identification of Antiviral Candidates against Coxsackievirus A16

Use of Viral Entry Assays and Molecular Docking Analysis for the Identification of Antiviral Candidates against Coxsackievirus A16

Published on: July 15, 2019

相关概念视频

Receptor Downregulation in MVBs

Receptor Downregulation in MVBs

Multivesicular bodies (MVBs) are mature endosomes that sort ubiquitinated proteins and then fuse with lysosomes to degrade the sorted proteins. Epidermal growth factor (EGF) and its receptor (EGFR) form a complex that can be internalized through endocytosis, sorted into an MVB, and later degraded.
The EGFR can initiate signaling pathways that lead to cell proliferation, migration, and differentiation. Overexpression of EGFR stimulates cells to proliferate. Excessive EGFR...

Leaky Scanning

Leaky Scanning

During most eukaryotic translation processes, the small 40S ribosome subunit scans an mRNA from its 5' end until it encounters the first start AUG codon. The large 60S ribosomal subunit then joins the smaller one to initiate protein synthesis. The location of the translation initiation is largely determined by the nucleotides near the start codon as there may be multiple translation initiation sites present on the mRNA. Marilyn Kozak discovered that the sequence RCCAUGG (where R...