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一个侧流装置,用于快速检测Scedosporium物种

Genna E Davies¹, Christopher R Thornton^1,2

¹ISCA Diagnostics Ltd., B12A, Hatherly Laboratories, Prince of Wales Road, Exeter EX4 4PS, UK.

Diagnostics (Basel, Switzerland)

|April 26, 2024

概括

一种新的快速侧流装置 (LFD) 测试可以在30分钟内检测出Scedosporium真菌感染. 这种敏感的测试使用单克隆抗体来识别人血清中的Scedosporium特定生物标志物,有助于更快的诊断.

科学领域:

医学真菌学医学真菌学
诊断微生物学诊断微生物学
免疫诊断免疫诊断免疫诊断免疫诊断

背景情况:

斯基多斯波里亚物种是人类显著的病原体,导致各种感染.
目前用于Scedosporium感染的诊断方法是缓慢的和侵入性的.
对于Scedosporium,急需快速和灵敏的诊断工具.

研究的目的:

开发一种快速和灵敏的诊断测试,用于发现Scedosporium感染.
在人血清中检测Scedosporium特定的生物标志物.

主要方法:

使用Scedosporium特异性IgG1单克隆抗体 (mAb),HG12开发侧流装置 (LFD) 测试.
该LFD的目标是通过Scedosporium hyphae分泌的细胞外多糖体 (EPS) 抗原.
测试与人体血清的兼容性,并确定关键Scedosporium物种的检测极限 (LOD).

主要成果:

开发的Scedosporium特定的LFD (ScedLFD) 测试在30分钟内提供pmoL/L灵敏度的结果.
该测试可以准确地检测人体血清中的S. apiospermum,S. aurantiacum和S. boydii的EPS生物标志物.
获得的LOD大约为S. apiospermum的0.81 ng/mL,S. aurantiacum的0.94 ng/mL,S. boydii的1.95 ng/mL.

结论:

该ScedLFD测试提供了一种新的,快速和敏感的方法来检测Scedosporium感染.
这种诊断工具有可能显著改善与Scedosporium有关的疾病的管理.
该测试可以检测多种负责人类感染的Scedosporium物种.

关键词:

这就是Scedosporium.生物标志物生物标志物与酶相关的免疫吸收剂测定.侧流装置的侧流装置是这是一种单克隆抗体.这种病名为scedosporiosis.

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