来自Scedosporium apiospermum菌细胞的细胞外囊:对于真菌与宿主相互作用的含义
Ana Carolina Aor1,2, Leandro S Sangenito1,3, Thaís P Mello1
1Departamento de Microbiologia Geral, Instituto de Microbiologia Paulo de Góes (IMPG), Centro de Ciências da Saúde (CCS), Universidade Federal do Rio de Janeiro (UFRJ), Rio de Janeiro 21941-902, RJ, Brazil.
Journal of fungi (Basel, Switzerland)
|April 26, 2024
概括
来自真菌Scedosporium apiospermum的细胞外囊泡 (EVs) 被识别和描述. 这些真菌EV携带抗原分子并通过增加真菌与宿主细胞的关联并表现出毒性来促进病原发生.
科学领域:
- 菌类学 菌类学是指菌类学.
- 细胞生物学 细胞生物学
- 免疫学 免疫学 免疫学
背景情况:
- 细胞外囊泡 (EVs) 在真菌中促进宏分子运输,但它们在线状真菌中的作用仍未得到充分探索.
- 斯基多斯波里亚 (Scedosporium apiospermum) 是一种具有临床意义的机会性,多药耐药的丝状真菌.
研究的目的:
- 为了净化和描述从Scedosporium apiospermum mycelia中的EV.
- 调查这些EVs在真菌病原发生中的潜在作用.
主要方法:
- 传输电子显微镜和光散射用于EV的表征.
- 生物化学分析 (脂质,碳水化合物,蛋白质含量) 和SDS-PAGE用于EV组成.
- 免疫洗,ELISA和免疫细胞化学检测抗原分子.
- 在体外细胞毒性测定使用哺乳动物细胞和Galleria mellonella.
主要成果:
- 确定了Scedosporium apiospermum EVs的平均直径为179.7nm,具有结构稳定性.
- EVs含有脂质,碳水化合物和蛋白质 (20-118 kDa),包括由特定抗体检测到的抗原分子.
- EVs对巨细胞和肺上皮细胞具有极小的细胞毒性,但对Galleria mellonella幼虫具有高度毒性.
- 用EVs进行预处理增强了S. apiospermum conidia与哺乳动物细胞的关联.
结论:
- 这项研究提供了第一份关于Scedosporium apiospermum.filamentous形式的EVs的报告.
- 菌EVs通过调节宿主细胞相互作用并表现出毒性,在病变发生过程中发挥作用.
- 在了解和对抗S. apiospermum感染方面,EVs是潜在的目标.
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