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在奶牛中对格兰正和格兰阴性乳腺感染进行不同的免疫控制

Giulio Curone¹, Joel Filipe¹, Alessia Inglesi¹

¹Dipartimento di Medicina Veterinaria e Scienze Animali, University of Milan, 26900 Lodi, Italy.

Veterinary sciences

|April 26, 2024

概括

在牛奶中研究了牛乳腺炎免疫力. 牛奶对黄金葡萄球菌的杀伤活性高于大肠杆菌,与NAGase水平和品种耐药性有关.

科学领域:

兽医免疫学兽医免疫学
乳制品科学乳制品科学
牛的健康问题

背景情况:

牛乳炎是乳制品行业的重要经济问题,导致生产损失和治疗费用.
了解导致乳腺内感染易感性和耐药性的先天免疫机制至关重要,但仍然不完整.

研究的目的:

调查牛脱脂牛奶的先天免疫反应,重点关注对抗格拉姆阳性和格拉姆阴性病原体的杀菌活动.
探索不同牛品种的杀菌活性,N-乙-β-D-葡萄糖胺酶 (NAGase) 水平,哺乳期和乳腺炎耐药性之间的关系.

主要方法:

从两个母牛群 (耐乳腺炎和易受乳腺炎的品种) 采集了无细胞牛脱脂奶和母乳样本,分别采集在乳房的不同阶段.
细胞度分析用于量化对培养的*大肠杆菌* (克拉姆阴性) 和*黄金色杆菌* (克拉姆阳性) 菌株的杀菌活性.
在牛奶和胆乳样本中测量了N-乙-β-D-葡萄糖胺酶 (NAGase) 的活性.

主要成果:

在牛奶和母乳样本中,杀死细菌的活性明显高于对*S. aureus*而不是对*E. coli*.
据称抗乳腺炎品种的牛奶中观察到更高的杀菌活性和NAGase水平.
无论是杀死金黄色菌的*活动还是NAGase水平,都与哺乳期的进展有负相关性.

结论:

牛奶对格拉姆阳性 (*S. aureus*) 和格拉姆阴性 (*E. coli*) 病原体具有不同的天生的免疫活性.
牛奶中的NAGas活性是杀死细菌能力的潜在指标,可能与乳腺炎耐药性有关.
哺乳期影响细菌杀死活动和NAGase水平,表明乳腺免疫力有动态变化.

关键词:

在NAGase中使用NAGase.杀死细菌的活动.奶牛牛奶牛牛牛牛牛牛牛牛牛牛牛牛牛牛牛牛牛牛牛牛牛牛牛天生的免疫反应.乳腺腺腺体乳腺体乳腺炎是一种乳腺炎.

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