相关实验视频

Updated: Jun 16, 2026

Detection of SARS-CoV-2 Receptor-Binding Domain Antibody using a HiBiT-Based Bioreporter

Detection of SARS-CoV-2 Receptor-Binding Domain Antibody using a HiBiT-Based Bioreporter

Published on: August 12, 2021

一个基于纳米盘的宿主受体生物传感器平台,用于检测特定病毒及其变体

Jin Yoo¹, Inkyoung Park², Sanghee Jwa³

¹School of Chemical and Biological Engineering, Institute of Chemical Processes, Seoul National University, Seoul 08826, Republic of Korea.

ACS applied materials & interfaces

|April 26, 2024

概括

这项研究开发了一种使用碳纳米管场效应晶体管上的ACE2受体嵌入纳米盘的新型传感器. 传感器可以检测SARS-CoV-2及其变体,具有高度的灵敏度和选择性,甚至可以检测突变病毒.

科学领域:

生物技术是生物技术.
纳米技术纳米技术
病毒学病毒学

背景情况:

主体受体对于病毒进入至关重要,可以用于病毒检测.
病毒发生突变以逃避抗体,但通常保留相同的细胞进入途径.
ангиотензин转化酶2 (ACE2) 是SARS-CoV-2进入的关键受体.

研究的目的:

开发一种用于敏感和选择性检测SARS-CoV-2及其变体的新型传感器.
调查ACE2受体嵌入纳米盘 (ND) 对病毒检测的实用性.
评估传感器的性能,包括灵敏度,选择性和长期功能.

主要方法:

一个碳纳米管场效应晶体管 (CNT-FET) 传感器的制造.
使用大肠杆菌表达系统生产和净化ACE2受体.
将ACE2集成到用于传感器制造的纳米盘 (ND) 平台中.
测试传感器与含有尖端蛋白的伪型病毒 (PVs) 的相互作用.

主要成果:

基于ACE2 ND的传感器检测到SARS-CoV-2及其变体的度低至10^2 PFU/mL.
传感器表现出极好的选择性,可以区分SARS-CoV-2变种 (Omicron,Delta) 和其他病毒 (ZIKA,MERS-CoV).
与可溶性ACE2相比,ACE2 ND在两周内表现出大约49%的优越长期功能.

结论:

基于宿主受体的传感器,特别是CNT-FET上的ACE2 ND,提供了一种高度敏感和选择性的检测病毒变异的方法.
这种方法为识别已知和潜在的新型病毒提供了一个强大的工具,有助于预防流行病.
纳米盘平台增强了受体的稳定性和功能性,提高了传感器的性能.

关键词:

在ACE2受体.在 CNT-FET 传感器上.在 COVID-19 疫情中,这就是SARS-CoV-2病毒.主体受体受体的宿主受体是什么一个纳米光盘.变种变种的变种

更多相关视频

Engineering Antiviral Agents via Surface Plasmon Resonance

Engineering Antiviral Agents via Surface Plasmon Resonance

Published on: June 14, 2022

A "Plug-And-Display" Nanoparticle Vaccine Platform Based on Outer Membrane Vesicles Displaying SARS-CoV-2 Receptor-Binding Domain

A "Plug-And-Display" Nanoparticle Vaccine Platform Based on Outer Membrane Vesicles Displaying SARS-CoV-2 Receptor-Binding Domain

Published on: July 25, 2022

相关概念视频

Microbial Biosensors

Microbial Biosensors

Microbial biosensors are analytical devices that utilize living microbes to detect specific substances through measurable signals. These devices consist of two main components: biosensing organisms and signal-transducing elements. Biosensing organisms, such as Escherichia coli or Saccharomyces cerevisiae, are typically housed in multiwell plates connected to transducers, enabling rapid, real-time detection of target analytes.Signal Generation MechanismWhen a target analyte—such as...

Rapid Identification of Pathogens

Rapid Identification of Pathogens

MALDI-TOF MS has transformed clinical microbiology by offering a rapid and reliable method for pathogen identification. The traditional approach to microbial identification typically involves time-consuming culture techniques and biochemical tests, which can delay the initiation of appropriate antimicrobial therapy. MALDI-TOF MS avoids these delays by using characteristic ribosomal protein mass patterns of microbial cells, enabling accurate species-level identification within minutes.Principle...

Automated Microbial Diagnostics

Automated Microbial Diagnostics

Automated diagnostic analyzers have transformed clinical microbiology by providing rapid and reliable methods for pathogen identification and antibiotic susceptibility testing. Among these systems, the Vitek 2 is widely used because it automates the traditionally labor-intensive processes of microbial identification (ID) and antibiotic susceptibility testing (AST), delivering standardized and timely results that are essential for effective patient care.Microbial Identification with ID CardsThe...