相关实验视频

Updated: Jun 27, 2025

Microfocus X-ray CT microCT Imaging of Actinia equina Cnidaria, Harmothoe sp. Annelida, and Xenoturbella japonica Xenacoelomorpha

Microfocus X-ray CT microCT Imaging of Actinia equina Cnidaria, Harmothoe sp. Annelida, and Xenoturbella japonica Xenacoelomorpha

Published on: August 6, 2019

光体的扫描电子显微镜:说明性地图集

Tigran P Norekian¹, Leonid L Moroz^2,3

¹Whitney Laboratory for Marine Biosciences University of Florida, St. Augustine, FL, USA. tigran@whitney.ufl.edu.

Methods in molecular biology (Clifton, N.J.)

|April 26, 2024

概括

扫描电子显微镜 (SEM) 协议被优化用于研究微妙的凝. 这项研究揭示了多样化的纤维状结构和复杂的解剖学ctenophores,适用于其他基底无脊椎动物.

科学领域:

海洋生物学海洋生物学
动物学动物学
显微镜的使用方法

背景情况:

海 (ctenophores) 是生态重要的一种海洋无脊椎动物.
它们的柔软,状体对传统扫描电子显微镜 (SEM) 构成重大挑战.
对类超结构的理解有限,这阻碍了与其他基底甲类动物进行比较研究.

研究的目的:

开发和验证强大的扫描电子显微镜 (SEM) 协议,用于微解剖学研究ctenophores.
研究状结构,受体类型和肌肉架构的超结构多样性,在选定的ctenophore物种.
为研究其他软体基底无脊椎动物类提供可适应的SEM方法.

主要方法:

使用优化软组织固定和脱水技术准备样本.
用于SEM成像的临界点干燥和喷雾涂层的应用.
对Pleurobrachia bachei和Beroe abyssicola进行了详细的显微镜检查.

主要成果:

成功地实现了ctenophore表面超结构的高分辨率成像.
在主要的功能系统中记录了状结构的惊人的多样性.
获得了对不同受体类型和复杂肌肉结构的新见解.
开发的协议被证明是可视化微细解剖细节的有效方法.

结论:

优化的SEM协议克服了ctenophore微解剖学的主要方法障碍.
这项研究揭示了光体中前所未有的超结构细节,突出了它们的生物复杂性.
这些方法为鱼和其他基底双边动物的比较超结构研究提供了宝贵的资源.

关键词:

博林诺普西斯 (Bolinopsis) 是一种植物.西里亚 (Cilia) 是一个小体.这就是Ctenophora.发展发展发展发展发展消化系统的消化系统.进化进化进化进化进化进化进化这就是 Mnemiopsis.肌肉肌肉肌肉肌肉这些神经元是神经元.腹肌病是什么意思?接收器接收器接收器

更多相关视频

Array Tomography Workflow for the Targeted Acquisition of Volume Information using Scanning Electron Microscopy

Array Tomography Workflow for the Targeted Acquisition of Volume Information using Scanning Electron Microscopy

Published on: July 15, 2021

Preparation of Prokaryotic and Eukaryotic Organisms Using Chemical Drying for Morphological Analysis in Scanning Electron Microscopy SEM

Preparation of Prokaryotic and Eukaryotic Organisms Using Chemical Drying for Morphological Analysis in Scanning Electron Microscopy SEM

Published on: January 7, 2019

相关概念视频

Overview of Microscopy Techniques

Overview of Microscopy Techniques

The early pioneers of microscopy opened a window into the invisible world of microorganisms. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes that leveraged nonvisible light, such as fluorescence microscopy that uses an ultraviolet light source and electron microscopy that uses short-wavelength electron beams. These advances significantly improved magnification, image resolution, and contrast. By comparison, the...

Electron Microscope Tomography and Single-particle Reconstruction

Electron Microscope Tomography and Single-particle Reconstruction

Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...

Scanning Electron Microscopy

Scanning Electron Microscopy

A scanning electron microscope (SEM) is used to study the surface features of a sample by using an electron beam that scans the sample surface in a two-dimensional manner. Typically, areas between ~1 centimeter to 5 micrometers in width can be imaged. SEM can be used to image bacteria, viruses, tissues as well as larger samples like insects. Conventional SEM gives a magnification ranging from 20X to 30,000X and spatial resolution of 50 to 100 nanometers.
Fundamental Principles
Accelerated...

Overview of Electron Microscopy

Overview of Electron Microscopy

The wavelengths of visible light ultimately limit the maximum theoretical resolution of images created by light microscopes. Most light microscopes can only magnify 1000X, and a few can magnify up to 1500X. Electrons, like electromagnetic radiation, can behave like waves, but with wavelengths of 0.005 nm, they produce significantly greater resolution up to 0.05 nm as compared to 500 nm for visible light. An electron microscope (EM) can create a sharp image that is magnified up to 2,000,000X.

Studying the Cytoskeleton

Studying the Cytoskeleton

The cytoskeletal architecture can be studied using different microscopic and biochemical techniques. Electron microscopy was instrumental in discovering the cytoskeletal architecture around the 1960s, which allowed obtaining structural information at a high-resolution level. However, the sample preparation procedure often limits this ability in biological samples. Several protocols have been developed over the years to optimize sample preparation. In one of the protocols known as rotary...

Transmission Electron Microscopy

Transmission Electron Microscopy

In 1931, physicist Ernst Ruska—building on the idea that magnetic fields can direct an electron beam just as lenses can direct a beam of light in an optical microscope—developed the first prototype of the electron microscope. This development led to the development of the field of electron microscopy. In the transmission electron microscope (TEM), electrons are produced by a hot tungsten element and accelerated by a potential difference in an electron gun, which gives them up to 400...