在Ctenophores中的DNA甲基化
Emily C Dabe1,2, Andrea B Kohn1, Leonid L Moroz3,4
1Whitney Laboratory for Marine Biosciences, University of Florida, St. Augustine, FL, USA.
Methods in molecular biology (Clifton, N.J.)
|April 26, 2024
概括
本研究提出了一种优化的全基因组双硫酸盐测序方法,用于无脊椎动物甲基组分析. 新的协议可以在脆弱的海洋动物 (如藻类) 中进行强大的DNA甲基化映射,揭示动态表观基因组模式.
科学领域:
- 基因组学就是基因组学.
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
- 海洋生物学 海洋生物学
背景情况:
- 基因甲基化是跨物种的关键表观基因调节剂,影响基因表达和进化.
- 全基因组双硫酸盐测序 (WGBS) 提供了DNA甲基化模式 (甲基组) 的单核酸解析.
- 现有的WGBS协议针对脊椎动物进行了优化,对无脊椎动物的适应性有限.
研究的目的:
- 开发和优化WGBS在非双边元生动物中的图书馆构建方法,特别是Ctenophore Mnemiopsis leidyi.
- 通过创建无脊椎动物甲基组参考,使物种之间的比较表观基因组研究成为可能.
- 为了研究动态DNA甲基化变化对刺激的反应.
主要方法:
- 优化了对脆弱的海洋无脊椎动物进行二硫酸盐测序库的构建.
- 集成的spike-in基因组DNA控制器,以准确测量甲基化转换效率.
- 聚合双硫酸盐转换反应以增加测序库产量和独特的映射率.
主要成果:
- 在全动物甲基组中成功检测到CpG,CHG和CHH位点的5-甲基细胞素 (5-mC).
- 使用circos图形可视化甲基化数据.
- 在受控和受伤条件下观察到与内素,毒素和神经相关的基因的DNA甲基化模式的变化.
结论:
- 开发的WGBS方法有效地从非双边元动物中产生高质量的甲基组.
- 这种方法有助于研究多样化和脆弱的海洋生物中的表观基因组调节.
- 该方法可以很容易地适应其他海洋无脊椎动物的表观基因组生产.
更多相关视频
07:16Rapid and Efficient Spatiotemporal Monitoring of Normal and Aberrant Cytosine Methylation within Intact Zebrafish Embryos
Published on: August 18, 2022
1.4K
07:50Immunohistochemical Detection of 5-Methylcytosine and 5-Hydroxymethylcytosine in Developing and Postmitotic Mouse Retina
Published on: August 29, 2018
8.9K
相关概念视频
01:46Epigenetic Regulation
31.0K
Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.
31.0K
03:17Inheritance of Chromatin Structures
6.2K
Epigenetics is the study of inherited changes in a cell's phenotype without changing the DNA sequences. It provides a form of memory for the differential gene expression pattern to maintain cell lineage, position-effect variegation, dosage compensation, and maintenance of chromatin structures such as telomeres and centromeres. For example, the structure and location of the centromere on chromosomes are epigenetically inherited. Its functionality is not dictated or ensured by the underlying...
6.2K
02:59Animal Mitochondrial Genetics
7.6K
Among all the organelles in an animal cell, only mitochondria have their own independent genomes. Animal mitochondrial DNA is a double-stranded, closed-circular molecule with around 20,000 base pairs. Mitochondrial DNA is unique in that one of its two strands, the heavy, or H, -strand is guanine rich, whereas the complementary strand is cytosine rich and called the light, or L, -strand. Compared to nuclear DNA, mitochondrial DNA has a very low percentage of non-coding regions and is marked by...
7.6K
02:30Histone Variants at the Centromere
4.3K
Histone variants are the histone proteins with structural and sequence variations. These variants may be regarded as “mutant” forms that replace their canonical histone counterparts in the nucleosomes. Specific post-translational modifications on the histone variants enable further chromatin complexity and regulate tissue-specific gene expression. The most common histone variants are from histone H2A, H2B, and linker histone H1 families. However, several variants of histone H3...
4.3K
02:38Heterochromatin
12.5K
The extent of chromatin compaction can be studied by staining chromatin using specific DNA binding dyes. Under the microscope, the dense-compacted regions that take up more dye are called heterochromatin. Heterochromatin is further classified into two forms – constitutive heterochromatin and facultative heterochromatin.
Constitutive heterochromatin: It is a highly compact region of chromatin that is mostly concentrated in the centromere and telomere. Unlike euchromatin, the amino acid at...
Constitutive heterochromatin: It is a highly compact region of chromatin that is mostly concentrated in the centromere and telomere. Unlike euchromatin, the amino acid at...
12.5K