视觉神经回路发展的转录控制由GS homeobox 1 1
Alexandra R Schmidt1, Haiden J Placer1, Ishmael M Muhammad1
1Department of Biology, West Virginia University, Morgantown, West Virgina, United States of America.
PLoS genetics
|April 26, 2024
概括
转录因子gsx1对于斑马鱼视觉回路的发展至关重要. 失去gsx1会损害视网膜结节细胞轴突的向和捕捉猎物等视觉行为.
科学领域:
- 神经科学是一个神经科学.
- 发展生物学 发展生物学
- 遗传学 是一个遗传学.
背景情况:
- 转录因子调节基因表达和神经电路组合.
- 在发育中的视觉系统中存在homeobox转录因子gsx1,但其功能尚不清楚.
- 视网膜质细胞 (RGC) 轴突在大脑中形成不同的树木化场 (AF),调解视觉行为.
研究的目的:
- 研究gsx1在斑马鱼视觉回路和相关行为发展中的作用.
- 了解gsx1如何影响RGC轴突向和前性AFs的形成.
主要方法:
- 使用了gsx1斑马鱼突变体,免疫组织化学和转基因线.
- 检查了RGC轴突在前膜 (Pr) 和光膜 (TeO) 中的向.
- 对特定的神经元群体进行定时激光切除.
主要成果:
- gsx1对于 Pr 中的膀性谷氨酸转运体表达是必不可少的,但不是总体的神经元数量.
- gsx1突变体表现出视力受损,Pr和TeO中的RGC轴突体积减少,并且没有AF7.
- 激光切除证实Tg ((slc17a6b:DsRed) 阳性细胞是AF7形成的必要条件.
结论:
- gsx1在视觉系统内建立神经元身份和功能神经回路方面发挥着关键作用.
- 这项研究涉及gsx1在特定的视觉行为的发展,如食,通过其在AF7形成中的作用.
相关概念视频
Transcription
Overview
Transcription is the process of synthesizing RNA from a DNA sequence by RNA polymerase. It is the first step in producing a protein from a gene sequence. Additionally, many other proteins and regulatory sequences are involved in the proper synthesis of messenger RNA (mRNA). Regulation of transcription is responsible for the differentiation of all the different types of cells and often for the proper cellular response to environmental signals.
Transcription Can Produce Different Kinds...
Transcription is the process of synthesizing RNA from a DNA sequence by RNA polymerase. It is the first step in producing a protein from a gene sequence. Additionally, many other proteins and regulatory sequences are involved in the proper synthesis of messenger RNA (mRNA). Regulation of transcription is responsible for the differentiation of all the different types of cells and often for the proper cellular response to environmental signals.
Transcription Can Produce Different Kinds...
Combinatorial Gene Control
Combinatorial gene control is the synergistic action of several transcriptional factors to regulate the expression of a single gene. The absence of one or more of these factors may lead to a significant difference in the level of gene expression or repression.
The expression of more than 30,000 genes is controlled by approximately 2000-3000 transcription factors. This is possible because a single transcription factor can recognize more than one regulatory sequence. The specificity in gene...
The expression of more than 30,000 genes is controlled by approximately 2000-3000 transcription factors. This is possible because a single transcription factor can recognize more than one regulatory sequence. The specificity in gene...
Master Transcription Regulators
Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
Transcription
Transcription is the synthesis of RNA from a DNA sequence by RNA polymerase. It is the first step in producing a protein from a gene sequence. Additionally, many other proteins and regulatory sequences are involved in correctly synthesizing messenger RNA (mRNA). Transcriptional regulation is responsible for the differentiation of different types of cells and often for the proper cellular response to environmental signals.
Transcription Can Produce Different Kinds of RNA Molecules
In eukaryotes,...
Transcription Can Produce Different Kinds of RNA Molecules
In eukaryotes,...
Regulation of Expression Occurs at Multiple Steps
Gene expression can be regulated at almost every step from gene to protein. Transcription is the step that is most commonly regulated. This involves the binding of proteins to short regulatory sequences on the DNA. This association can either promote or inhibit the transcription of a gene associated with the respective sequence.
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...
Master Transcription Regulators
Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...


