素H3K27脱甲基酶SlJMJ3调节番茄果实成熟的过程
Zhiwei Li1,2,3, Jing Zeng1,2,3, Yijie Zhou1,4
1State Key Laboratory of Plant Diversity and Specialty Crops and Guangdong Provincial Key Laboratory of Applied Botany, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, China.
Plant physiology
|April 26, 2024
概括
番茄果实成熟是由SlJMJ3调节的,这是一种消除H3K27me3标记的基因组脱甲酶. 这种表观遗传调节器通过激活关键的成熟相关基因来加速成熟.
科学领域:
- 植物生物学 植物生物学
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
- 分子生物学分子生物学
背景情况:
- 基因组 lysine (K) 脱甲基酶4 (KDM4/JHDM3) Jumonji 域含有脱甲基酶 (JMJs) 子家族参与植物发育.
- JMJs在肉质水果成熟中的作用基本上是未知的.
研究的目的:
- 研究KDM4/JHDM3家族成员SlJMJ3在番茄果实成熟中的功能.
- 阐明SlJMJ3调节成熟的表观遗传机制.
主要方法:
- 在番茄中识别SlJMJ3作为H3K27me3脱甲基酶.
- 在SlJMJ3过度表达和功能丧失突变体中分析水果成熟表型.
- ChIP-seq用于识别SlJMJ3向基因和结合基因.
- 对成熟相关基因的基因表达分析.
主要成果:
- 在番茄中,SlJMJ3充当H3K27me3脱甲基酶.
- 过度表达SlJMJ3会加速水果的成熟,而功能丧失则会延迟成熟.
- SlJMJ3直接与成熟相关基因 (含有CTCTGYTY基因) 的促进者结合,并通过降低H3K27me3水平来调节它们的表达.
结论:
- SlJMJ3在调节番茄果实成熟方面发挥着至关重要的作用.
- SlJMJ3通过从与成熟相关的基因中去除H3K27me3标记来作为表观遗传调节器,从而调节成熟过程.
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