对于基因素-2 电机的双重和对立的作用,KIF17,在依赖的小脑发育中
Bridget Waas1, Brandon S Carpenter2, Nicole E Franks1
1Department of Cell and Developmental Biology, University of Michigan, Ann Arbor, MI, 48109, USA.
Science advances
|April 26, 2024
概括
通过调节 (HH) 信号通路,KIF17对小脑发育至关重要. 这种运动蛋白在产生HH的普金涅细胞和对HH反应的小脑颗粒神经元原始体中具有相反的作用,影响细胞增殖.
科学领域:
- 神经科学是一个神经科学.
- 细胞生物学 细胞生物学
- 发展生物学 发展生物学
背景情况:
- 包括KIF3A和KIF3B在内的Kinesin-2电机对于纤维生成和刺 (HH) 信号传输至关重要.
- 另一种基因素-2电机KIF17在HH信号传递和小脑发育中的特定作用仍然未被探索.
研究的目的:
- 调查KIF17对依赖HH的大脑小脑发育的贡献.
- 阐明KIF17在产生HH的普金尼细胞和对HH反应的小脑颗粒神经元 (CGNP) 中的不同作用.
主要方法:
- 在小鼠中利用生殖线KIF17删除来评估小脑发育.
- 采用Purkinje细胞特异性和CGNP特异性的KIF17删除策略.
- 分析了Sonic HH (SHH) 蛋白质水平和GLI转录因子处理.
主要成果:
- 生殖线KIF17的缺失导致小脑低成形,降低了CGNP的增殖,并且由于较低的Sonic HH (SHH) 蛋白质,降低了HH通路活性.
- 普尔金耶细胞特异的KIF17删除部分模仿了生殖线删除表型.
- 特定于CGNP的KIF17删除导致CGNP增殖和HH目标基因表达的增加,这与改变的GLI转录因子处理有关.
结论:
- 已确定KIF17是HH依赖小脑发育的关键调节剂.
- 在Purkinje细胞 (HH生产者) 和CGNP (HH响应者) 中,KIF17表现出双重和对立的功能.
- 这些发现凸显了KIF17在调节小脑生长和HH信号传递方面的复杂作用.
相关概念视频
The Movement of Organelles and Vesicles
4.4K
In eukaryotic cells, cytoskeletal filaments such as actin, microtubules, and intermediate filaments form a mesh-like cytoskeletal network. These filaments serve as tracks for transporting cellular cargo. Specialized motor proteins use the chemical energy stored in adenosine triphosphate (ATP) for this transport. During interphase, microtubules are polarized, with the plus-end towards the cell periphery and the minus-end towards the cell center. Two microtubule-associated motor proteins,...
4.4K
Hedgehog Signaling Pathway
7.3K
The Hedgehog gene (Hh) was first discovered due to its control of the growth of disorganized, hair-like bristles phenotype in Drosophila, much like hedgehog spines. Hh plays a crucial role in the development of organs and the maintenance of homeostasis in both invertebrates and vertebrates. However, while Drosophila has only one Hh protein, mammals have multiple functional Hedgehog proteins - Sonic (Shh), Desert (Dhh), and Indian Hedgehog (Ihh). All of these homologous proteins have adapted to...
7.3K
Microtubule Associated Motor Proteins
7.9K
Eukaryotic cells have different motor proteins for transporting various cargo within the cell. These motor proteins differ based on the filament they associate with, the direction they move within the cell, and the type of cargo they transport. Motor proteins that associate with microtubules are known as microtubule-associated motor proteins. There are two families of microtubule-associated motor proteins —Kinesins and Dyneins. Both these proteins assist in the transport of cellular...
7.9K
Spindle Assembly
3.6K
Spindle assembly occurs through three, often coexisting, pathways – the centrosome-mediated pathway, the chromatin-mediated pathway, and the microtubule-mediated pathway – collectively contributing to form a robust spindle apparatus.
In most cells, centrosomes are the primary microtubule nucleation centers. In the centrosome-mediated pathway, the G2-prophase transition triggers centrosome maturation and increased microtubule nucleation. Progressive nucleation results in a...
In most cells, centrosomes are the primary microtubule nucleation centers. In the centrosome-mediated pathway, the G2-prophase transition triggers centrosome maturation and increased microtubule nucleation. Progressive nucleation results in a...
3.6K
Role of Septins
1.7K
Septins are the recently discovered fourth major protein component of the cytoskeleton, along with microfilaments, microtubules, and intermediate filaments. These proteins can associate with other cytoskeletal filaments and carry out varied roles or can be free-floating in the cytoplasm.
Cellular Functions of Septins
Recent studies have revealed the multifaceted roles of septins in various cellular processes such as cytokinesis, ciliogenesis, and neurogenesis. Septins act as scaffolds and...
Cellular Functions of Septins
Recent studies have revealed the multifaceted roles of septins in various cellular processes such as cytokinesis, ciliogenesis, and neurogenesis. Septins act as scaffolds and...
1.7K
Mechanism of Ciliary Motion
3.7K
The ciliary structures were first seen in 1647 by Antonie Leeuwenhoek while observing the protozoans. In lower organisms, these appendages are responsible for cell movement, while in higher organisms, these appendages help in the movement of the extracellular fluids within the body cavities.
The cilia are made up of microtubules in a 9+2 arrangement, with nine microtubule doublet ring bundles, surrounding a pair of central singlet microtubule bundles. The doublet microtubule bundles are...
The cilia are made up of microtubules in a 9+2 arrangement, with nine microtubule doublet ring bundles, surrounding a pair of central singlet microtubule bundles. The doublet microtubule bundles are...
3.7K


