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更薄并不总是更好:优化冷灯泡对子图片平均的优化

Maarten W Tuijtel1, Sergio Cruz-León2, Jan Philipp Kreysing1,3

  • 1Department of Molecular Sociology, Max Planck Institute of Biophysics, Max-von-Laue-Straße 3, 60438 Frankfurt am Main, Germany.

Science advances
|April 26, 2024
PubMed
概括

低温电子断层扫描 (cryo-ET) 数据质量取决于样品厚度. 最佳的拉美拉厚度高达180nm不会影响分辨率,表面的损伤仅限于30nm.

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Electron Microscope Tomography and Single-particle Reconstruction01:07

Electron Microscope Tomography and Single-particle Reconstruction

Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...