一个无仪器的,集成的微型平台,用于在资源有限的环境中快速和多重检测乳制品造
Nan Wang1, Xiaoyun Sun1, Juan Zhang1
1Institute of Quality Standard & Testing Technology for Agro-Products, Key Laboratory of Agro-product Quality and Safety, Chinese Academy of Agricultural Sciences, Beijing, 100081, China.
Biosensors & bioelectronics
|April 26, 2024
概括
一个新的便携式微流体设备,部门自动驾驶微流体 (SDM),可以快速地在现场检测牛奶造. 这项技术在不到一个小时内准确识别特定物种的成分,确保乳制品质量.
科学领域:
- 食品科学与技术 食品科学与技术
- 分析化学 分析化学
- 生物技术是生物技术.
背景情况:
- 特殊乳制品容易被更便宜的牛奶来源改.
- 目前的检测方法是实验室制定的,耗时的,需要专家操作.
- 需要快速的现场检测技术来确保乳制品的真实性.
研究的目的:
- 开发和验证一个便携式的,集成的微流体平台,用于同时检测多种牛奶物种特定组件.
- 为检测乳制品改提供高效,准确和现场解决方案.
主要方法:
- 发展部门自动驾驶微流体 (SDM),一个封闭的微流体系统,集成核酸提取,净化,循环介导同热放大 (LAMP) 和侧流带 (LFS) 检测.
- 利用磁力和毛细血管作用进行自动样本处理,消除了外部.
- 由移动电池供电,可完全携带和在现场操作.
主要成果:
- SDM平台实现了8种常见的牛奶物种特定成分的无污染视觉检测.
- 乳制品成分的检测到1%的含量在1小时内实现.
- 与实时PCR相比,对35个商业样本的验证显示出100%的特异性和准确性.
结论:
- SDM平台为乳制品行业提供了一种高效,方便和准确的工具.
- 允许在生产或销售点进行快速现场测试,以实时监控质量.
- 促进早期风险识别和防止不符合标准的乳制品进入市场.
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