休息和激活的牛中性粒细胞和乙氨基粒细胞在它们对上腺激素激动剂的反应上有所不同
Angela Howell1, Natasa Arsic2, Philip Griebel3
1Vaccine and Infectious Disease Organization (VIDO), University of Saskatchewan, 120-Veterinary Road, Saskatoon, SK S7N 5E3, Canada; Global Institute for Food Security (GIFS), University of Saskatchewan, 421 Downey Rd, Saskatoon, SK S7N 4L8, Canada.
牛中性粒细胞和乙氨基粒细胞是多态核细胞 (PMN) 的类型,对上腺激素激动剂有不同的反应. 了解这些差异对于研究牛的先天免疫防御是至关重要的.
科学领域:
- 兽医免疫学 兽医免疫学
- 具有天生的免疫力.
- 细胞免疫学 细胞免疫学
背景情况:
- 多态核细胞 (PMN) 对于天生的免疫力至关重要,但压力会改变它们的功能.
- 牛PMN中的上腺素受体 (AR) 表达和功能尚不清楚.
- 中性粒细胞和异osinophils,不同的PMN亚群,表现出差异性的AR基因表达.
研究的目的:
- 开发一种方法来区分牛中性粒细胞和乙中性粒细胞.
- 分析这些亚群体对上腺激素激动剂的反应.
- 为了比较内源和合成上腺激动剂对牛PMN的影响.
主要方法:
- 开发了一种流细胞测量技术,以区分牛中性粒细胞和乙素粒细胞.
- 在激活时评估CD11b,L-选择蛋白和CD44表达的变化.
- 对比休息和激活细胞对上腺素,上腺素和合成AR激动剂的反应.
主要成果:
- 在激活后,中性粒细胞和氨酸细胞显示了CD11b,L-选择蛋白和CD44的不同表达变化.
- 这两种细胞类型在用上腺素和上腺素刺激后表现出iROS,CD44和L-选择素表达的明显变化.
- 休息中性粒细胞对上腺素和上腺素都表现出促炎反应,而休息中性粒细胞只对上腺素做出反应.
结论:
- 牛中性粒细胞和乙氨基粒细胞对上腺激动剂的反应不同,改变了参与免疫监测和炎症的蛋白质.
- 这些差异性反应与两种细胞类型之间AR基因表达的已知差异相关.
- 单独分析中性粒细胞和性粒细胞亚种群对于准确调查牛群中AR激动剂效应至关重要.
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