通过离心隔离分离色谱法分离 ochratoxins
Zsófia Hegedüs1, Csilla Gömöri2, Mónika Varga2
1Department of Microbiology, Faculty of Science and Informatics, University of Szeged, Közép fasor 52, Szeged H-6726, Hungary; Doctoral School in Biology, Faculty of Science and Informatics, University of Szeged, Szeged, Hungary.
Journal of chromatography. A
|April 26, 2024
概括
研究人员开发了一种高效的离心分离色谱 (CPC) 方法来净化毒素 (OT). 这种技术成功地从微生物培养物中分离了毒素A (OTA) 和毒素B (OTB),用于进一步分析.
科学领域:
- 自然产品 化学 化学
- 分析化学 分析化学
- 生物技术是生物技术.
背景情况:
- 毒素 (OTs) 是具有重大毒理影响的真菌毒素.
- 目前用于OT的净化方法在常规分析中可能是低效的.
- 菌株 A. albertensis SZMC 2107 产生 ochratoxins,需要有效的隔离技术.
研究的目的:
- 开发一种高效的液体-液体色谱法,用于常规的 ochratoxin 净化.
- 优化离心分离染色学 (CPC) 系统,以分离甲胺A (OTA) 和甲胺B (OTB).
- 从微生物液体培养物中分离并确认OT的纯度.
主要方法:
- 离心分离染色学 (CPC) 用于分离.
- 为了确定最佳的双相系统,各种三级溶剂系统进行了选.
- 为了高效的CPC分离,选择了带有酸的六/i-propanol/水系统.
- 高性能液体染色学 (HPLC) 用于初始提取物分析和纯度确认.
主要成果:
- 一种高效的CPC方法是使用/i-propanol/水 (35:15:50) 溶剂系统与0.1%的酸建立的.
- 从1升发酵中成功分离了Ochratoxin A (OTA) 和Ochratoxin B (OTB).
- 隔离的OTA达到96.3%的纯度,OTB达到72.8%的纯度.
- 隔离的OT的身份和纯度得到了确认.
结论:
- 开发的CPC方法提供了一种高效和有效的手段,用于常规净化毒素.
- 这种方法适用于隔离OT用于随后的分析或毒理学研究.
- CPC提供了一种可行的替代方案,用于从复杂的生物矩阵中净化 ochratoxins.
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