VvERF117通过对抗氧化基因BAS1的直接调节,积极调节葡萄的耐寒性
Beibei Li1, Yushuang Zang1, Changze Song1
1College of Enology, Northwest A&F University, Yangling, Shaanxi 712100.
International journal of biological macromolecules
|April 26, 2024
概括
乙烯反应因子VvERF117通过提高抗氧化能力,提高葡萄的耐寒性. 这涉及调节VvBAS1基因,这有助于减轻活性氧物种的损害.
科学领域:
- 植物生物学 植物生物学
- 分子生物学分子生物学
- 压力生理学 压力生理学
背景情况:
- 寒冷压力对葡萄种植构成重大威胁,影响品质,产量和分布.
- 已知乙烯反应因子 (ERF) 在寒冷应激反应中起作用,但许多调节机制仍然未被阐明.
- 了解涉及寒冷耐受性的特定ERF对于开发弹性葡萄品种至关重要.
研究的目的:
- 在葡萄中识别和表征冷反应基因.
- 阐明VvERF117在调节寒冷耐受性的作用.
- 研究VvERF117介导的抗寒性背后的分子机制.
主要方法:
- 对VvERF117.7的基因鉴定和表征
- 过度表达和沉默的VvERF117在转基因阿拉比多普西斯,葡萄和葡萄叶.
- 对耐寒性,基因表达和抗氧化酶活性 (POD) 的分析.
- 鉴定VvERF117.7的直接点基因
主要成果:
- 过度表达VvERF117在各种植物组织中增强了耐寒性,同时沉默增加了敏感性.
- VvERF117提高了与压力相关的基因的表达,包括直接标VvBAS1,它编码了2-Cys过氧化酶 (POD).
- 过度表达VvERF117增加了POD活性,并在寒冷压力下降了过氧化 (H2O2) 水平,表明抗氧化能力增强.
结论:
- VvERF117作为葡萄中耐寒性的积极调节剂.
- 通过提高抗氧化能力,VvERF117增强了抗寒能力,部分是通过调节VvBAS1和随后缓解反应性氧物种.
- 这些发现为葡萄中寒冷应激反应的分子基础提供了洞察力,并为培育耐寒品种提供了潜在的目标.
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