反向电子转移:通过外源CO2与生物炭协同作用而调节的新型无氧甲基生成途径
Yang Qiu1, Jingxin Zhang2, Yen Wah Tong3
1China-UK Low Carbon College, Shanghai Jiao Tong University, Shanghai 200240, China.
Bioresource technology
|April 26, 2024
概括
外源的二氧化碳 (CO2) 和生物炭通过促进反向电子转移 (RET),增加甲生产和酸降解来增强无氧消化. 这种协同作用加强了热力学可行性,并指导了甲基生成.
科学领域:
- 生物地质化学生物地质化学
- 环境微生物学 环境微生物学
- 生物技术是生物技术.
背景情况:
- 无氧消化 (AD) 面临着由酸积累和碳排放带来的挑战.
- 合成微生物群落利用反向电子转移 (RET) 在乙烯生成中产生不利的氧化还原反应.
- 在AD中RET的机制和调节尚未完全理解.
研究的目的:
- 为了研究外源二氧化碳 (CO2) 如何调节无氧消化过程中的RET.
- 评估二氧化碳和生物炭对AD过程的协同效应.
- 阐明CO2介导的RET背后的生物能量和微生物机制.
主要方法:
- 评估生物炭对二氧化碳可溶性增强的作用.
- 测量累积的甲产量和酸降解率.
- 分析生物能量变化 (吉布斯自由能量) 和微生物群体的转变 (酶活性,细菌群体,同位素追踪).
主要成果:
- 生物炭将二氧化碳的溶解度最大化至25.8 mmol/L,增强了二氧化碳的作用.
- 二氧化碳与生物炭相结合,显著提高了甲产量和酸降解.
- 添加二氧化碳降低了吉布斯自由能量到-87kJ/mol,改善了热力学有利性.
- 通过增加形式脱酶活性和H2/形式利用细菌的丰富,包括Methanospirillum hungatei,证明了RET促进.
- 同位素标记 (5% 13CH4) 证实了CO2在定向甲生成中的作用.
结论:
- 外源CO2,特别是当被生物炭溶解时,在无氧消化过程中有效促进RET.
- 二氧化碳生物炭协同作用通过改进的生物能源增强了甲生产和有机酸降解.
- 这项研究扩大了对二氧化碳和生物炭在阿尔茨海默病中的作用的理解,突出了它们优化RET过程的潜力.
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