解读FUS::DDIT3表达和瘤微环境在状脂肪肉瘤发展中的作用
Parmida Ranji1,2, Emma Jonasson1, Lisa Andersson1
1Sahlgrenska Center for Cancer Research, Department of Laboratory Medicine, Institute of Biomedicine, Sahlgrenska Academy at University of Gothenburg, Gothenburg, Sweden.
Journal of translational medicine
|April 26, 2024
概括
具有FUS::DDIT3表达的myxoid脂肪瘤细胞创建了自己的细胞外. 这种融合瘤基因,不仅仅是瘤微环境,驱动了这种肉瘤中特有的前脂肪细胞表型.
科学领域:
- 在瘤学瘤学.
- 生物化学 生物化学
- 细胞生物学 细胞生物学
背景情况:
- 状脂肪瘤 (MLS) 具有独特的瘤微环境.
- FUS::DDIT3融合瘤基因是MLS的特征.
- 微环境和FUS::DDIT3在MLS发展中的作用尚未完全理解.
研究的目的:
- 研究MLS瘤微环境和FUS::DDIT3表达的功能贡献.
- 开发一个实验模型来研究MLS中的无细胞微环境.
主要方法:
- 开发了一个使用脱细胞化患者衍生的异种移植瘤 (支架) 的模型.
- 通过质谱测量特征化了脚手架.
- 重新填充的支架与表达FUS::DDIT3或非表达肉瘤细胞,其次是组织学和RNA测序.
主要成果:
- 脱细胞化的MLS支架保留了完整的结构和多样化的蛋白质.
- FUS::DDIT3表达减少了细胞增殖和支架的侵入性.
- 微环境和FUS::DDIT3都影响了细胞相互作用,染色质重塑,免疫反应和新陈代谢.
- FUS::DDIT3表达是前脂肪细胞表型的主要驱动因素.
结论:
- 实验模型为详细的瘤微环境研究提供了新的途径.
- FUS::DDIT3表达的瘤细胞可以积极塑造它们的细胞外.
- 在定义MLS表型方面,FUS::DDIT3起着至关重要的作用.
关键词:
细胞外矩阵是细胞外矩阵.在FET融合瘤基因.FUS::DDIT3 在线播放微观环境是一个微观环境.状脂肪肉瘤 (Myxoid liposarcoma) 是一种癌症.脚手架 脚手架是一个脚手架.更多相关视频
09:52A Mimic of the Tumor Microenvironment: A Simple Method for Generating Enriched Cell Populations and Investigating Intercellular Communication
Published on: September 20, 2016
10.3K
07:20A Novel Stromal Fibroblast-Modulated 3D Tumor Spheroid Model for Studying Tumor-Stroma Interaction and Drug Discovery
Published on: February 28, 2020
9.9K
相关概念视频
02:17The Tumor Microenvironment
6.6K
Every normal cell or tissue is embedded in a complex local environment called stroma, consisting of different cell types, a basal membrane, and blood vessels. As normal cells mutate and develop into cancer cells, their local environment also changes to allow cancer progression. The tumor microenvironment (TME) consists of a complex cellular matrix of stromal cells and the developing tumor. The cross-talk between cancer cells and surrounding stromal cells is critical to disrupt normal tissue...
6.6K
02:23Abnormal Proliferation
4.5K
Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
4.5K