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应用Cas12j用于Streptomyces编辑

Lee Ling Tan¹, Elena Heng¹, Chung Yan Leong²

¹Institute of Molecular and Cell Biology (IMCB), Agency for Science, Technology and Research (A*STAR), 61 Biopolis Drive, Proteos #07-06, Singapore 138673, Singapore.

|April 27, 2024

概括

这项研究引入了超紧的Cas12j用于Streptomyces的基因组编辑,增强了天然产品的发现. Cas12j显示出激活静态基因集群的潜力,扩大了这些微生物的CRISPR工具箱.

科学领域:

微生物学微生物学
分子生物学分子生物学
生物技术是生物技术.

背景情况:

克里斯普尔-卡斯系统加速了Streptomyces中的天然产品发现.
SpCas9的取决于菌株的效率和非目标效应限制了基因组编辑.
多样化的Cas蛋白工具箱对于更广泛的Streptomyces遗传操纵至关重要.

研究的目的:

为了研究Acidaminococcus sp.的疗效. Cas12j用于Streptomyces中的基因组编辑.
评估Cas12j在激活静音生物合成基因集群 (BGCs) 的潜力.
扩大可用的CRISPR-Cas工具用于actinomycete基因组工程.

主要方法:

探索Cas12j用于各种Streptomyces菌株的基因组编辑.
评估Cas12j的转换和编辑效率.
在激活BGC时,Cas12j与SpCas9和Cas12a的性能进行比较.

主要成果:

Cas12j显示了比SpCas9.9更高的转换效率.
与Streptomyces sp.中的Cas12a相比,Cas12j显示了BGC激活效率的提高. A34053. 一个有价值的人
Cas12j编辑效率低于Cas12a和Cas9.9之前报告的效率.

结论:

Cas12j扩展了CRISPR-Cas编辑基因组中的基因组编辑.
卡斯12j显示了在Streptomyces中激活沉默的BGC的潜力,有助于天然产品的合成.
一个全面的遗传工具箱对于成功编辑各种Streptomyces菌株的基因组至关重要.

关键词:

这就是CRISPR-Cas.在Cas12j-2中.这种病名为Streptomyces.基因组编辑基因组编辑发现自然产品的发现.

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