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在膀癌中,tRNA衍生碎片作为生物标志物
Olaf Strømme1, Kathleen A Heck1, Gaute Brede1
1Department of Clinical and Molecular Medicine, Norwegian University of Science and Technology, 7034 Trondheim, Norway.
Cancers
|April 27, 2024
概括
研究人员确定了细胞外囊泡 (EV) 中的转移RNA衍生碎片 (tRF) 作为膀癌 (BC) 诊断和监测无复发生存的潜在非侵入性生物标志物.
科学领域:
- 在瘤学瘤学.
- 分子生物学分子生物学
- 生物化学 生物化学
背景情况:
- 膀癌 (BC) 诊断依赖于侵入性囊镜,促使人们寻找非侵入性生物标志物.
- 尿液和血液等体液中的细胞外囊泡 (EV) 含有有价值的分子载荷,包括RNA.
- 转移RNA衍生碎片 (tRFs) 是一种在EV中发现的小型非编码RNA的类.
研究的目的:
- 研究tRFs在EV中的潜力,作为膀癌的非侵入性生物标志物.
- 为了比较非肌肉侵入性BC患者与非癌症患者 (NCPs) 的tRF表达特征.
- 评估tRFs对于诊断BC和预测无复发生存的有用性.
主要方法:
- 小RNA下一代测序在41名BC患者和15名NCP的尿液和血清EV上进行.
- 采集了手术前和后的样本 (膀瘤的顺尿路切除).
- 差异表达分析确定了特定的tRFs (DEtRFs),并研究了它们的基因组对齐.
主要成果:
- 在尿液EV中确定了14种差异表达的tRF (DEtRF),将T1阶段BC患者与NCPs进行比较.
- 在T1阶段患者手术前和后的样本比较时,在血清中发现了6个DEtRF.
- 在将手术前样本与NCPs进行比较时,在血清上位物中发现了四种DEtRF.
结论:
- 尿液和血清EV中的特定tRF显示出作为膀癌诊断的非侵入性生物标志物的潜力.
- 这些tRF可能有助于监测疾病复发和预测无复发生存率.
- 已识别的DEtRF与参与癌症发展的基因组区域保持一致,这表明其具有功能相关性.
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