变化表达和功能活动的myosinII异型在扩大的前列腺多可塑性前列腺
Xiao Wang1, Weixiang He2, Hui Chen1
1Department of Urology, Renmin Hospital of Wuhan University, Wuhan 430071, China.
Journal of personalized medicine
|April 27, 2024
概括
良性前列腺增生 (BPH) 涉及光滑肌肉肌肉蛋白 (SMM) 和非肌肉肌肉蛋白 (NMM) 异型的变化. 肌肉蛋白表达的这些变化有助于改变前列腺光滑肌肉收缩性和下泌尿道症状 (LUTS).
科学领域:
- 泌尿器科 泌尿器科 泌尿器科 泌尿器科
- 分子生物学分子生物学
- 病理生理学 病理生理学
背景情况:
- 良性前列腺增生 (BPH) 是老年男性中普遍存在的疾病,其特征是下泌尿道症状 (LUTS).
- 前列腺光滑肌肉 (SM) 收缩,受光滑肌肉肌肉蛋白质 (SMM) 和非肌肉肌肉蛋白质 (NMM) 异型的影响,是BPH发病的一个关键因素.
- 在前列腺MS收缩和BPH发育中肌素的作用背后的精确机制需要进一步阐明.
研究的目的:
- 与正常前列腺组织相比,研究SMM和NMM异型在良性前列腺增生 (BPH) 组织中的差异表达.
- 分析这些髓异形变化的影响,前列腺光滑肌的收缩性.
- 基于改变的肌肉蛋白表达,探索LUTS/BPH的潜在治疗点.
主要方法:
- 前列腺组织从BPH患者和正常捐赠者收集.
- 使用一种丸激素诱导的BPH大鼠模型.
- 竞争性RT-PCR被用于评估SMM异型表达.
- 进行了器官浴研究,以评估人类前列腺条纹的收缩性.
主要成果:
- 与正常组织相比,人类BPH组织显示SM-1和肌轻链-17b (MLC17b) 异型的表达增加.
- 在BPH患者中观察到前列腺肌重链 (MHC) 表达 (mRNA和蛋白质) 的显著下降.
- 在BPH组织中,在mRNA和蛋白质水平上检测到非肌肉肌肉胺重链B (NMMHC-B) 的升级表达.
- 来自BPH患者的前列腺条纹在体外表现出较慢的强力收缩.
结论:
- 在BPH患者的前列腺扩大时,MHC表达显著减少,伴随着SM-1,MLC17b和NMMHC-B的水平升高.
- 这些SMM和NMM异型的修改可能会影响前列腺光滑肌的强力收缩特性.
- 了解这些肌蛋白变化,可以了解LUTS/BPH的发展情况,并可能确定新的治疗策略.
更多相关视频
相关概念视频
02:23Abnormal Proliferation
4.5K
Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
4.5K
01:15Overview of Myosin Structure and Function
4.3K
Myosins are a family of molecular motor proteins, first identified in the skeletal muscles, where they are responsible for muscle contraction. Along with their role in muscle contraction, these proteins also play a role in the intracellular transport of molecules and vesicles. There are twenty-four classes of myosins based on their domain sequence and organization. Of the twenty-four, six classes (Myosin I, Myosin II, Myosin V, Myosin VI, Myosin VII, and Myosin X) have been well...
4.3K
01:18Role of Myosin in Cell Migration
2.3K
Myosins are multimeric motor proteins involved in various cellular processes such as migration, adhesion, and proliferation. Myosin II is the most common type in animal cells, which binds and cross-links actin filaments.
Myosin II is a hexamer comprising two heavy chains with globular heads and coiled-coil tails, two regulatory light chains, and two essential light chains. The ATPase sites on the myosin heads hydrolyze ATP, and the released phosphate generates the force for contraction....
Myosin II is a hexamer comprising two heavy chains with globular heads and coiled-coil tails, two regulatory light chains, and two essential light chains. The ATPase sites on the myosin heads hydrolyze ATP, and the released phosphate generates the force for contraction....
2.3K