相关实验视频

Updated: Jun 27, 2025

Author Spotlight: THP-1 Macrophage Response to LPS/ATP — Unveiling the Pyroptosis, Apoptosis, and Necroptosis Spectrum

Author Spotlight: THP-1 Macrophage Response to LPS/ATP — Unveiling the Pyroptosis, Apoptosis, and Necroptosis Spectrum

Published on: May 3, 2024

有机阻燃剂TPP诱导的人类角膜上皮细胞通过卡斯帕斯依赖的线粒体通路的亡

Chen Chen¹, Daolei Cui¹, Jingya Li¹

¹Yunnan Province Innovative Research Team of Environmental Pollution, Food Safety and Human Health, Institute of Environmental Remediation and Human Health, School of Ecology and Environment, Southwest Forestry University, Kunming 650224, China.

International journal of molecular sciences

|April 27, 2024

概括

三酸 (TPP),一种有机酸盐阻燃剂 (OPFR),会损害人类角膜上皮细胞 (HCEC). 通过线粒体通路,TPP降低了细胞活力,并诱导了细胞亡,突出了OPFR的健康风险.

科学领域:

环境毒理学环境毒理学
细胞生物学细胞生物学
眼部毒理学眼部毒理学

背景情况:

三酸 (TPP) 是一种常见的有机酸阻燃剂 (OPFR),在环境和人类中发现.
关于TPP暴露于人角膜上皮的健康风险的数据有限.

研究的目的:

研究TPP对人类正常角膜上皮细胞 (HCEC) 的细胞毒性.
阐明TPP诱导的HCEC损伤背后的分子机制,包括亡和线粒体功能障碍.

主要方法:

HCEC暴露于不同度的TPP.
评估了细胞活力,形态,细胞亡标志物 (Cyt c,Caspase-9,Caspase-3,Bcl-2,Bax) 和线粒体膜潜力.

主要成果:

TPP以度依赖的方式降低了HCEC的活力 (IC50 = 220μM).
在剂量取决的方式中,TPP诱导了显著的亡并降低了线粒体膜潜力.
观察到关键的亡相关基因mRNA水平的变化.

结论:

在HCEC中,TPP表现出细胞毒性,导致细胞亡和线粒体功能障碍.
卡斯巴酶依赖的线粒体通路与TPP诱导的HCEC亡有关.
这些发现强调了与OPFR暴露相关的潜在人类健康风险,特别是对角膜的暴露.

关键词:

这就是TPPTPPTPP.细胞亡是细胞的亡.基因表达的基因表达方式人类角膜上皮细胞线粒体亡途径的线粒体亡途径

更多相关视频

High Throughput SiRNA Screening for Chloropicrin and Hydrogen Fluoride-Induced Cornea Epithelial Cell Injury

High Throughput SiRNA Screening for Chloropicrin and Hydrogen Fluoride-Induced Cornea Epithelial Cell Injury

Published on: June 16, 2018

Evaluation of Caspase Activation to Assess Innate Immune Cell Death

Evaluation of Caspase Activation to Assess Innate Immune Cell Death

Published on: January 20, 2023

相关概念视频

The Extrinsic Apoptotic Pathway

The Extrinsic Apoptotic Pathway

The extrinsic apoptotic pathway is initiated when extracellular death-inducing signals, such as specific cytokines, activate the death receptors expressed on the cell surface. The immune cells involved in this pathway are natural killer cells (NK cells) and cytotoxic T-lymphocytes. NK cells are critical in innate immune response, while cytotoxic T-lymphocytes are associated with adaptive immune response. These cells recognize specific receptors expressed on the altered cells and activate...