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在Streptomyces物种中对抗微生物药物的代谢比较和分子网络

Bijaya Bahadur Thapa¹, Chen Huo², Rabin Budhathoki¹

¹Central Department of Chemistry, Tribhuvan University, Kirtipur 44618, Kathmandu, Nepal.

International journal of molecular sciences

|April 27, 2024

概括

来自尼泊尔土壤的四种Streptomyces物种产生了新的抗菌化合物. 这些强效的天然产品,包括deketopiperazines,对抗耐药细菌具有潜力,解决了对新抗生素的关键需求.

科学领域:

微生物学微生物学
自然产品化学自然产品化学
药物发现药物发现药物发现

背景情况:

耐多药 (MDR) 微生物对全球健康构成重大威胁,需要发现新型抗菌剂.
杆菌种类是生物活性二次代谢物的丰富生产者,具有已被证明的抗菌性质.

研究的目的:

从尼泊尔的土壤样本中分离和识别Streptomyces物种.
评估这些分离物对关键人类病原体的抗菌活性.
用先进的分析技术来描述有前途的菌株的二次代谢组.

主要方法:

使用形态学和16S rRNA基因测序来隔离和识别Streptomyces物种.
对一个由格拉姆阳性和格拉姆阴性细菌组成的小组进行抗微生物敏感性测试.
乙酸乙烯提取物通过液体染色学-并联质谱学 (LC-MS/MS) 和全球天然产品社会分子网络 (GNPS) 进行分析.

主要成果:

在尼泊尔土壤中分离了四种Streptomyces菌株 (PC1,BT1,BT2,BT3).
杆菌物种PC1对测试的病原体表现出最高的抗菌活性.
在LC-MS/MS和GNPS分析中,发现了37种代谢物,主要是二甲基,其中包括4种潜在的新化合物和已知的表面素和瓦利诺米.

结论:

尼泊尔土壤拥有具有显著抗菌潜力的Streptomyces物种.
已识别的代谢物,特别是新型二基托皮佩拉,代表了开发新抗生素的有希望的候选者.
使用LC-MS/MS和GNPS的新陈代谢分析是有效的发现和表征Streptomyces的天然产品.

关键词:

抗生素抗生素是一种抗生素.质谱测量质谱测量质谱测量质谱测量质量测量质谱测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量代谢生物组的代谢生物组微生物天然产品中的微生物天然产品.

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