抗微生物水凝的抗菌和溶血活性利用不动化的抗微生物
Edvin Blomstrand1,2, Elin Posch1,2, Annija Stepulane1,3
1Department of Chemistry and Chemical Engineering, Chalmers University of Technology, Kemigården 4, SE-412 96 Göteborg, Sweden.
International journal of molecular sciences
|April 27, 2024
概括
将抗微生物 (AMP) 附在水凝上可以提高稳定性,并消除溶血活性. 与自由相比,与表面结合的AMP显示出对Pseudomonas aeruginosa的效果改变.
科学领域:
- 生物材料科学 生物材料科学
- 传染病研究 传染病研究
- 类化学 类化学
背景情况:
- 抗微生物 (AMP) 在治疗细菌感染方面表现有前途,但在稳定性和毒性方面面临挑战.
- 对材料的共价附着是一种提高AMP稳定性和降低毒性的策略.
- 最佳的附着方法和AMP的支持作用仍在调查中.
研究的目的:
- 为了评估六种不同AMP的抗菌疗效和血液溶解活性,这些AMP被共地附着在两性水凝上.
- 为了比较表面结合的AMP与其免费对应物的表现,对抗金黄色葡萄球菌和Pseudomonas aeruginosa.
- 调查表面粘附对AMP稳定性和毒性的影响.
主要方法:
- 六种不同的AMP (四种合成变体,Piscidin 1和Omiganan) 与一个有序的两性水凝的共价附着.
- 对 Staphylococcus aureus 和 Pseudomonas aeruginosa 的抗菌活性在溶液和水凝结合状态的评估.
- 评估自由和凝结合的AMP的溶血活性.
主要成果:
- 对黄金葡萄球菌的抗菌疗效与自由和表面结合的AMP相似.
- 针对Pseudomonas aeruginosa的活性在自由AMP和附着在水凝中的AMP之间有显著差异.
- 所有测试的AMP在溶液中都表现出溶解性活性,在水凝附着时完全取消,使它们变得非溶解性.
结论:
- 通过消除溶血活性,AMP与水凝的共价附着显著提高了它们的安全性.
- AMPs的表面固定可以调节它们的抗菌谱,特别是对抗具有挑战性的病原体,如Pseudomonas aeruginosa.
- 凝支持的AMP是开发更稳定,更安全的抗微生物疗法的有希望的策略.
更多相关视频
相关概念视频
01:23Antimicrobial Proteins
984
Antimicrobial proteins are important components of the immune system. They aid the body in combating pathogens by either killing them directly or hindering their replication processes. Four main types of antimicrobial substances are interferons, the complement system, iron-binding proteins, and antimicrobial proteins.
Interferons
Interferons (IFNs) are proteins produced by lymphocytes, macrophages, and fibroblasts infected with viruses. While IFNs cannot prevent viruses from entering and...
Interferons
Interferons (IFNs) are proteins produced by lymphocytes, macrophages, and fibroblasts infected with viruses. While IFNs cannot prevent viruses from entering and...
984
01:18Surface Membrane Barriers
1.1K
The skin and mucous membranes serve as the primary line of defense against pathogens by providing both physical and chemical protection. These barriers are essential in preventing the entry and establishment of microbes, thereby maintaining the integrity of the host.
The outer layer of the skin, the epidermis, is a robust barrier comprising layers of closely packed keratinized cells. This dense arrangement prevents microbes from penetrating the body. The periodic shedding of epidermal cells...
The outer layer of the skin, the epidermis, is a robust barrier comprising layers of closely packed keratinized cells. This dense arrangement prevents microbes from penetrating the body. The periodic shedding of epidermal cells...
1.1K