MYB转录因子GmMYB78负面调节了大豆中的Phytophthora sojae抗性
Hong Gao1, Jia Ma1, Yuxin Zhao1
1Soybean Research Institute of Northeast Agricultural University/Key Laboratory of Soybean Biology of Chinese Education Ministry, Harbin 150030, China.
International journal of molecular sciences
|April 27, 2024
概括
豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆
科学领域:
- 植物病理学 植物病理学
- 分子生物学分子生物学
- 植物与微生物的相互作用
背景情况:
- 由Phytophthora sojae引起的Phytophthora根腐烂是大豆的一个重要疾病.
- 了解大豆耐药机制对于疾病管理至关重要.
- GmAP2此前已被确定为增强大豆对P. sojae.e.的敏感性.
研究的目的:
- 阐明GmMYB78在大豆对Phytophthora sojae的反应中的作用.
- 调查GmMYB78在疾病易感性或耐药性中的功能背后的分子机制.
- 确定GmMYB78.8的上游和下游调节器.
主要方法:
- 基因表达分析 (转录组,qPCR).
- 转基因大豆毛发根中的基因过度表达和沉默.
- 对莉酸 (JA) 含量进行量化.
- 蛋白质与蛋白质相互作用和调节途径的识别.
主要成果:
- GmMYB78是由P. sojae诱导的,并作为抗性的负调节剂,增强了易感性.
- GmMYB78可以负面调节素酸 (JA) 信号通路和JA合成基因GmAOS1.1.
- GmMYB78影响了与病变发生相关的基因的表达.
- GmbHLH122作为GmMYB78的上游抑制剂,而GmbZIP25则是GmMYB78.8激活的下游标.
结论:
- GmMYB78通过抑制JA信号和与致病相关的基因表达,促进大豆对Phytophthora sojae的敏感性.
- GmbHLH122-GmMYB78-GmbZIP25调控级联参与大豆对P. sojae.的反应.
- GmMYB78是提高大豆对Phytophthora根腐蚀的抵抗力的潜在目标.
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