体内体内自由2+ 在细胞形成过程中的变化
Sharon Dewitt1, Joanna Green2, Iraj Laffafian2
1Biomaterials Group, School of Dentistry, College of Biological Life Sciences, Cardiff University, Cardiff CF14 4XY, UK.
International journal of molecular sciences
|April 27, 2024
概括
(Ca2+) 道在体上允许离子进入细胞质中. 这项研究揭示了在人类中性粒细胞的细胞化过程中出现的新型围体Ca2+信号.
科学领域:
- 细胞生物学 细胞生物学
- 分子生物学分子生物学
- 免疫学 免疫学 免疫学
背景情况:
- 消化细胞形成了消化细胞体,这是一个新型的器官,将细胞外和细胞内环境分开.
- 离子信号传递,特别是 (Ca2+),在血膜中至关重要,但在细胞体-细胞醇边界方面不太了解.
- 之前的研究集中在细胞形成期间的Ca2+流入,忽视了体内Ca2+动态.
研究的目的:
- 为了研究在人类中性粒细胞的 fagocytosis 和 phagosome 形成期间的 intra-phagosomal Ca2+ 含量的变化.
- 探索细胞/细胞质边界在Ca2+信号传递中的作用.
- 为了识别胞体上潜在的Ca2+通道.
主要方法:
- 测量体内Ca2+度,仔细控制pH值和氧化工件.
- 利用PKC-γ的YFP标记的C2域来监测Ca2+流入和膜转位.
- 观察细胞杯中的Ca2+动态以及细胞发生过程中的细胞.
主要成果:
- 排除的文物证实开放的Ca2+道在虫体.
- 有证据表明,Ca2+离子可以从体流入周围的细胞质中.
- YFP-PKC-γ转位表明Ca2+的流入最初是在细胞杯,然后是全球.
结论:
- 体具有功能性的Ca2+通道,可促进离子进入细胞质中.
- 确定了新的围体Ca2+信号事件和热点.
- 这些发现有助于我们更好地理解细胞信号通路中的Ca2+动态.
更多相关视频
11:47Real-time Live-cell Flow Cytometry to Investigate Calcium Influx, Pore Formation, and Phagocytosis by P2X7 Receptors in Adult Neural Progenitor Cells
Published on: April 3, 2019
9.2K
10:07"Phagosome Closure Assay" to Visualize Phagosome Formation in Three Dimensions Using Total Internal Reflection Fluorescent Microscopy TIRFM
Published on: August 26, 2016
6.5K
相关概念视频
Feedback Regulation of Calcium Concentration
3.4K
Calcium is an essential signaling molecule required for various cellular functions. Calcium pumps and ion channels on cell and organellar membranes, such as those on the endoplasmic reticulum (ER), regulate calcium concentrations inside the cell. They remain closed, keeping the cytosolic calcium levels low at a resting state.
Various transmembrane receptors, such as G protein-coupled receptors (GPCRs), elicit a response to extracellular signals by increasing cytosolic calcium. Activated GPCRs...
Various transmembrane receptors, such as G protein-coupled receptors (GPCRs), elicit a response to extracellular signals by increasing cytosolic calcium. Activated GPCRs...
3.4K
Calmodulin-dependent Signaling
5.1K
Calmodulin (CaM) is a calcium-binding protein in eukaryotes that controls various calcium-regulated cellular processes. It has four calcium-binding sites that bind calcium to form the calcium-calmodulin ( Ca2+-CaM) complex. GPCR stimulation increases the calcium levels in the cells that bind to CaM and induces a conformational change.
The Ca2+-CaM complex does not have enzymatic activity by itself. Instead, the complex binds downstream target proteins, including membrane proteins or enzymes,...
The Ca2+-CaM complex does not have enzymatic activity by itself. Instead, the complex binds downstream target proteins, including membrane proteins or enzymes,...
5.1K
Phagocytosis of Apoptotic Cells
3.8K
Cells undergoing apoptosis form apoptotic bodies that must be removed immediately to prevent inflammation, autoimmune diseases, and necrosis. Phagocytosis is carried out by professional phagocytes such as macrophages or immature dendritic cells. Non-professional phagocytes such as epithelial cells and fibroblasts also take part in this process; however, they are not as effective as professional phagocytes.
Normal cells contain receptors that prevent them from being recognized...
Normal cells contain receptors that prevent them from being recognized...
3.8K
Phagocytosis
80.0K
Cells pull particles inward and engulf them in spherical vesicles in an energy-requiring process called endocytosis. Phagocytosis (“cellular eating”) is one of three major types of endocytosis. Cells use phagocytosis to take in large objects—such as other cells (or their debris), bacteria, and even viruses.
80.0K
IP3/DAG Signaling Pathway
12.0K
Membrane lipids such as phosphatidylinositol (PI) are precursors for several membrane-bound and soluble second messengers. Specific kinases phosphorylate PI and produce phosphorylated inositol phospholipids. One such inositol phospholipids are the phosphatidylinositol-4,5 bisphosphate [PI(4,5)P2], present in the inner half of the lipid bilayer. Upon ligand binding, GPCR stimulates Gq proteins to turn on phospholipase Cꞵ. Activated phospholipase Cꞵ cleaves PI(4,5)P2 and...
12.0K
