相关实验视频

Updated: May 11, 2026

Evaluating the Effectiveness of Cancer Drug Sensitization In Vitro and In Vivo

Evaluating the Effectiveness of Cancer Drug Sensitization In Vitro and In Vivo

Published on: February 6, 2015

准结肠直肠癌:揭示西斯普拉丁和高THC大麻提取物的转录学影响

Viktoriia Cherkasova¹, Yaroslav Ilnytskyy¹, Olga Kovalchuk¹

¹Department of Biological Sciences, University of Lethbridge, Lethbridge, AB T1K 3M4, Canada.

International journal of molecular sciences

|April 27, 2024

概括

研究人员调查了高THC大麻提取物和西斯能否与结直肠癌协同作用. 相反,他们发现了一种对抗性相互作用,大麻提取物可能会阻碍西斯丁.

科学领域:

在瘤学瘤学.
药理学药理学是指药理学的学科.
分子生物学分子生物学

背景情况:

衍生化疗药物,如思是癌症治疗的主要支柱.
瘤对青的耐药性需要探索新的治疗策略和药物组合.
包括delta-9-tetrahydrocannabinol (THC) 在内的大麻素正在因其抗癌特性而受到研究.

研究的目的:

评估西斯普拉丁与高THC大麻提取物相结合对结直肠癌细胞系 (HT-29,HCT-116,LS-174T) 的潜在协同作用.
通过转录基因分析阐明基斯普拉丁与高THC大麻提取物之间的相互作用的分子机制.
了解这些组合如何影响癌细胞死亡,DNA修复和抗药性途径.

主要方法:

在实验室测试 cisplatin 和高THC 大麻提取物在结直肠癌细胞系.
对HT-29细胞系进行全面的转录组分析,以确定受影响的分子通路.
基因表达分析专注于细胞死亡,DNA修复和药物耐药性标记.

主要成果:

高THC大麻提取物和西斯的组合表明对测试的结直肠癌细胞系产生对抗作用.
转录组分析显示,对抗性可能与细胞死亡关键途径 (例如,BCL2,BAD,caspase10) 中的基因表达改变有关.
相互作用还影响了参与DNA修复 (Rad52) 和癌症抗药途径的基因.

结论:

高THC大麻提取物和西斯普拉丁在结直肠癌细胞系中表现出敌对的相互作用,与预期的协同效应相反.
观察到的对抗性与与亡,DNA修复和药物耐药性相关的基因转录的显著改变有关.
这些发现凸显了药物相互作用的复杂性,并表明高THC大麻可能不是适合用于结直肠癌的西斯普拉丁化疗的辅助药物.

关键词:

大麻提取物大麻提取物西斯普拉丁 (cisplatin) 是一种制剂.结肠直肠癌是什么意思德尔塔-9-四基大麻醇 (delta-9-tetrahydrocannabinol) 是一种大麻的化合物.

更多相关视频

Discovery of Driver Genes in Colorectal HT29-derived Cancer Stem-Like Tumorspheres

Discovery of Driver Genes in Colorectal HT29-derived Cancer Stem-Like Tumorspheres

Published on: July 22, 2020

Author Spotlight: Unveiling the Role of TMOD3 in Platinum Resistance and Immune Infiltration in Ovarian Cancer

Author Spotlight: Unveiling the Role of TMOD3 in Platinum Resistance and Immune Infiltration in Ovarian Cancer

Published on: August 2, 2024

相关概念视频

Targeted Cancer Therapies

Targeted Cancer Therapies

The targeted cancer therapies, also known as “molecular targeted therapies,” take advantage of the molecular and genetic differences between the cancer cells and the normal cells. It needs a thorough understanding of the cancer cells to develop drugs that can target specific molecular aspects that drive the growth, progression, and spread of cancer cells without affecting the growth and survival of other normal cells in the body.
There are several types of targeted therapies against specific...

Chemotherapy-Induced Nausea and Vomiting: Cannabinoids

Chemotherapy-Induced Nausea and Vomiting: Cannabinoids

Tetrahydrocannabinol (THC) is a phytocannabinoid that primarily interacts with the CB1 receptor, a type of G protein-coupled receptor (GPCR) predominantly in and around the chemoreceptor trigger zone (CTZ) and emetic center. THC also blocks the serotonin receptor activity in the dorsal vagal complex (DVC) by inhibiting serotonin release. THC exerts its anti-emetic effects through these interactions, which are beneficial for patients undergoing chemotherapy.
Two synthetic agonists of THC,...

Pharmacogenetics of Drug Targets: β₂-Adrenergic Receptors, Apo E, Thymidylate Synthase

Pharmacogenetics of Drug Targets: β₂-Adrenergic Receptors, Apo E, Thymidylate Synthase

Genetic polymorphisms in drug targets have emerged as critical determinants of interindividual variability in drug response and toxicity. Pharmacogenomic investigations increasingly focus on identifying these variations to personalize and optimize therapeutic interventions. A drug target may be a receptor, enzyme, or signaling protein involved in pharmacologic responses or disease-related pathways. While early pharmacogenetic studies focused primarily on drug metabolism, current research...