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转录基因变化和与新疆棕牛乳腺炎耐药性相关的监管网络
Dan Wang1, Haiyan Yang2, Shengchao Ma1
1College of Animal Science, Xinjiang Agricultural University, Urumqi 830091, China.
Genes
|April 27, 2024
概括
在新疆棕牛中发现乳腺炎耐药性的基因是基因改进的关键. 这项研究在高体细胞计数 (SCC) 牛中发现了更高的细胞因子水平,并确定了参与牛乳腺炎的关键基因和非编码RNA.
科学领域:
- 动物遗传学动物遗传学
- 兽医免疫学 兽医免疫学
- 分子生物学分子生物学
背景情况:
- 新疆棕牛表现出抗病能力和粗耐受性.
- 乳腺炎耐药性基因鉴定为牛的基因改进提供了一条新的途径.
- 了解乳腺炎的分子机制对于乳牛群的健康至关重要.
研究的目的:
- 为了研究不同体细胞计数 (SCC) 的新疆棕牛的血细胞因子水平.
- 用RNA测序识别新疆棕牛中与乳腺炎相关的差异表达基因和非编码RNA.
- 构建竞争的内源性RNA (ceRNA) 网络并阐明参与牛乳腺炎的关键调节途径.
主要方法:
- 在高和低SCCs的牛中量化血液中细胞因子水平 (IL-1β,IL-6,IL-10,TNF-α,TGF-β).
- 使用RNA测序 (RNA-seq) 对健康和受乳腺炎影响的牛进行外周血液的转录基因分析.
- 对mRNAs,lncRNAs和circRNAs的差异表达分析,随后进行途径丰富分析和ceRNA网络构建.
主要成果:
- 在高SCCs的牛中观察到血中细胞因子水平升高.
- 通过RNA-seq发现了大量的差异表达的mRNA,lncRNA和circRNA.
- 丰富的途径包括PI3K/Akt,焦点粘附,ECM受体相互作用,胆固醇突触和细胞因子/受体活性.
- 构造的ceRNA网络中的关键基因和ncRNAs表明在乳腺炎调节中的作用.
结论:
- 细胞因子概况和转录基因数据为新疆棕牛的乳腺炎病原体提供了洞察力.
- 鉴定出差异表达的基因和ncRNAs,特别是在ceRNA网络内,是基因改进和乳腺炎控制的潜在目标.
- PI3K-Akt信号通路和细胞粘附分子与牛乳腺炎的调节有关.
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