相关实验视频

Updated: Jun 27, 2025

Optimization and Comparative Analysis of Plant Organellar DNA Enrichment Methods Suitable for Next-generation Sequencing

Optimization and Comparative Analysis of Plant Organellar DNA Enrichment Methods Suitable for Next-generation Sequencing

Published on: July 28, 2017

一个高质量的组装和对 Actinidia macrosperma 线粒体的比较分析

Jiangmei Gong^1,2, Jun Yang³, Yan Lai^1,2

¹College of Horticulture, Fujian Agriculture and Forestry University, Fuzhou 350002, China.

|April 27, 2024

概括

我们对Actinidia macrosperma的线粒体基因组进行了测序,揭示了它的结构和遗传内容. 这项研究为Actinidia属研究和资源利用提供了必要的遗传数据.

科学领域:

植物基因组学植物基因组学
分子生物学分子生物学
药用植物学药用植物学

背景情况:

传统药用植物Actinidia macrosperma的线粒体基因组 (线粒体基因组) 在很大程度上没有表征.
了解线粒基因组对于植物遗传学研究和Actinidia属内的遗传资源管理至关重要.

研究的目的:

为了测序和描述Actinidia macrosperma的完整线粒体基因组.
分析它的组装,遗传内容,并识别重复的元素.
为了研究与 хлоропласт基因组共享的同源序列.

主要方法:

全基因组测序和组装的Actinidia宏观精子的线粒基因组.
生物信息分析用于识别基因,SSR和重复结构.
与 хлоропласт基因组进行比较分析.

主要成果:

阿克丁尼迪亚宏观精子线粒基因组是一个圆形分子,752,501 bp,GC含量为46.16%.
它包含63个基因 (39个蛋白质编码,23个tRNA,3个rRNA),63个SSR和许多重复序列.
在线粒基因组和叶绿体基因组之间发现了53个同类片段,其中最长的为4296 bp.

结论:

这是关于Actinidia macrosperma线粒体的第一份报告,提供了宝贵的遗传资源.
这些数据有助于在Actinidia属内进行遗传学分析.
它支持更广泛地利用这种药用植物物种的遗传资源.

关键词:

这种种子是Actinidia macrosperma.codon 的使用情况基基因组是如何形成的遗传学分析遗传学分析重复的元素是重复的元素.

更多相关视频

An Integrated Approach for Microprotein Identification and Sequence Analysis

An Integrated Approach for Microprotein Identification and Sequence Analysis

Published on: July 12, 2022

Comprehensive Workflow for the Genome-wide Identification and Expression Meta-analysis of the ATL E3 Ubiquitin Ligase Gene Family in Grapevine

Comprehensive Workflow for the Genome-wide Identification and Expression Meta-analysis of the ATL E3 Ubiquitin Ligase Gene Family in Grapevine

Published on: December 22, 2017

相关概念视频

Comparing Mitochondrial, Chloroplast, and Prokaryotic Genomes

Comparing Mitochondrial, Chloroplast, and Prokaryotic Genomes

The present-day mitochondrial and chloroplast genomes have retained some of the characteristics of their ancestral prokaryotes and also have acquired new attributes during their evolution within eukaryotic cells. Like prokaryotic genomes, mitochondrial and chloroplast genomes neither bind with histone-like proteins nor show complex packaging into chromosome-like structures, as observed in eukaryotes. Unlike mitotic cell divisions observed in eukaryotic cells, mitochondria and chloroplasts...

Genome Annotation and Assembly

Genome Annotation and Assembly

The genome refers to all of the genetic material in an organism. It can range from a few million base pairs in microbial cells to several billion base pairs in many eukaryotic organisms. Genome assembly refers to the process of taking the DNA sequencing data and putting it all back together in a correct order to create a close representation of the original genome. This is followed by the identification of functional elements on the newly assembled genome, a process called genome annotation.

Spindle Assembly

Spindle Assembly

Spindle assembly occurs through three, often coexisting, pathways – the centrosome-mediated pathway, the chromatin-mediated pathway, and the microtubule-mediated pathway – collectively contributing to form a robust spindle apparatus.
In most cells, centrosomes are the primary microtubule nucleation centers. In the centrosome-mediated pathway, the G2-prophase transition triggers centrosome maturation and increased microtubule nucleation. Progressive nucleation results in a...