两种赫索索载体对Magnaporthe oryzae中基质亲和性和致病性的影响
Tinghong Huang1, Dekang Guo1, Xiao Luo1
1National Key Laboratory of Green Pesticide/Key Laboratory of Natural Pesticide and Chemical Biology, Ministry of Education, South China Agricultural University, Guangzhou 510642, China.
Microorganisms
|April 27, 2024
概括
麦格纳波特或瑞泽 (Magnaporthe oryzae) 体载体 (HXT) 对于大米爆发至关重要. MoHXT2显著影响病原性,而MoHXT1不参与糖的运输或黑色素的产生.
科学领域:
- 植物病理学 植物病理学
- 分子生物学分子生物学
- 生物化学 生物化学
背景情况:
- 黑色素转运体 (HXT) 在真菌病原体Magnaporthe oryzae中对营养获取和信号传递至关重要.
- 了解HXT的功能是制定对抗米爆病的策略的关键.
研究的目的:
- 调查MoHXT1,MoHXT2和MoHXT3在Magnaporthe oryzae病原性和黑色素运输中的作用.
- 阐明这些黑色素载体之间的功能差异和调节相互作用.
主要方法:
- 使用CRISPR/Cas9基因编辑来创建MoHXT1-3删除突变.
- 酵母补充试验和Xenopus卵细胞电生理学被用来评估hexose亲和力.
- 分析了转录资料,以了解基因表达调节.
主要成果:
- 删除MoHXT1并没有影响黑色素的形成或黑色素的运输.
- 虽然MoHXT2与MoHXT3相比具有较低的赫索索亲和力,但对病原性的影响更大.
- 删除MoHXT2降低了MoHXT3的表达,而MoHXT3的淘汰会提高MoHXT2的转录.
- 发现一种MoHXT2变种无法进行体运输.
结论:
- MoHXT2在Magnaporthe oryzae的致病性中发挥着重要作用,可能是通过超出直接体运输的机制.
- MoHXT2和MoHXT3表达之间的相互作用突出了复杂的监管网络.
- 针对这些赫索索转运器提供了一种潜在的策略,以破坏碳营养供应和控制大米爆炸.
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