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全基因组测序和Streptococcus equi亚种的表型分析. 序列型147从中国分离出来的动物流行病

Yan Su¹, Zehua Zhang¹, Li Wang¹

¹Department of Microbiology and Immunology, College of Veterinary Medicine, Xinjiang Agricultural University, Urumqi 830052, China.

|April 27, 2024

概括

Streptococcus zooepidemicus 菌株 ZHZ 211 呈现出增强的毒性因子和抗生素耐药性. 基因组分析揭示了可移动的遗传元素,有助于其致病性,有助于理解这一重要的动物性传染病原体.

科学领域:

微生物学微生物学
基因组学就是基因组学.
兽医医学兽医医学兽医医学

背景情况:

Streptococcus equi 的一个亚种. 动物流行病菌 (S. zooepidemicus) 是一个重要的动物感染病原体.
越来越多的隔离率和耐药性突显了基因组研究的必要性.
S. zooepidemicus 在动物和人类中引起严重疾病.

研究的目的:

进行S. zooepidemicus菌株ZHZ 211.1.的深入基因组分析.
识别基因组岛屿,移动遗传元素和毒性/抗性基因.
为了表征S. zooepidemicus菌株ZHZ 211的表型.

主要方法:

关于S. zooepidemicus菌株ZHZ 211的全基因组测序.
与其他参考菌株进行基因组比较分析.
在体外表型特征,包括生长速度,抗生素耐药性和生物膜形成.

主要成果:

获得了高质量的2.18 Mb染色体,揭示了八个基因组岛屿.
菌株ZHZ 211具有众多的毒性因子,包括铁吸收系统,粘附因子,外酶和抗菌细胞基因.
获取的移动遗传元素原体Ph01含有两个氨基酶 (hyl) 基因;两个转子子和两个毒性 (virD4) 基因位于同一基因组岛上.
现型分析显示了更快的生长,对克拉里胺素,恩洛夫洛素和硫胺素的耐药性,以及增强的生物膜形成.

结论:

对S. zooepidemicus菌株ZHZ 211的基因组和表型表征扩大了对其致病性的理解.
移动遗传元素,特别是P01,在S. zooepidemicus的毒性中起着至关重要的作用.
鉴定到的抗性基因和增强的生物膜形成有助于该菌株的生存和临床意义.
对S. zooepidemicus分子致病机制的进一步研究是有必要的.

关键词:

Streptococcus equi 的一个亚种. 动物流行病病毒 (zooepidemicus)氨基酶基因是氨基酶的基因.预言预言预言预言进行全基因组测序.

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