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便采样对常规临床方法的影响,用于量化便中的六种短链脂肪酸,使用气相色谱-质谱法

Tarek Mahdi^1,2, Aurore Desmons³, Pranvera Krasniqi³

¹Hôpital Pitié Salpêtrière-Charles Foix, AP-HP, Service de Biochimie Endocrinienne et Oncologique, 75000 Paris, France.

|April 27, 2024

概括

短链脂肪酸 (SCFA) 对肠道健康至关重要. 解热化是处理便样本的最佳方法,以准确测量SCFA作为肠道健康的潜在生物标志物.

科学领域:

微生物学和生物化学
胃肠道学和营养学

背景情况:

短链脂肪酸 (SCFA) 是肠道细菌从未消化的碳水化合物中产生的关键代谢物.
SCFA,特别是黄油酸,对于结肠细胞的能量和维持肠道完整性至关重要.
异常的SCFA水平与IBD,IBS和结直肠癌等胃肠道疾病有关.

研究的目的:

适应和验证一种可靠的分析方法,用于量化便样本中的SCFA.
调查各种预分析便处理方法对SCFA测量的影响.
在临床实验室环境中建立SCFA分析的标准化协议.

主要方法:

液体-液体提取,其次是异布酸衍生物和气色谱-质谱 (GC-MS) 分析.
在新鲜便,乙醇/甲醇稀释剂,冷解便和RNAlater®保存样本中对SCFA量化进行比较.
分析前后研究,以评估方法性能和样本稳定性.

主要成果:

开发了一种经过验证的GC-MS方法,用于在便样本中准确量化SCFA.
解热化成为优越的便加工方法,确保可靠和可重复的SCFA测量.
该研究确定了用于一致的SCFA生物标志物分析的最佳预分析条件.

结论:

经过验证的GC-MS方法为临床诊断中SCFA分析提供了可靠的工具.
建议在便样本制备过程中进行冷化,以标准化SCFA测量.
准确的SCFA量化可以帮助理解肠道健康和诊断相关疾病.

关键词:

在GC-MS中使用GC-MS.我们的肠道微生物组.这是一个代谢组的代谢组.短链脂肪酸短链脂肪酸便抽样是指便的抽样.

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