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猪眼组织中的酸盐代谢使用¹⁵N-标记的酸盐补充剂来确定

Ji Won Park^1,2, Barbora Piknova^1,2, Khalid J Tunau-Spencer^1,2

¹Molecular Medicine Branch, National Institute of Diabetes and Digestive and Kidney Diseases, Bethesda, MD 20892, USA.

|April 27, 2024

概括

食中酸盐补充剂显著增加了猪眼组织中的酸盐和酸盐水平,包括水性和玻璃性幽默. 这表明在眼部条件下提高氧化水平的潜在治疗应用.

科学领域:

生理学生理学生理学
生物化学生物化学
眼科医生眼科眼科

背景情况:

饮食中的酸盐 (NO₃^-) 在体内转化为酸盐 (NO₂^-) 和氧化 (NO).
猪眼组织含有显著的酸盐和酸盐水平,角膜和膜将酸盐减少为酸盐.

研究的目的:

调查猪眼组织中的酸盐代谢和NO前体水平.
评估饮食中酸盐补充剂对眼睛中酸盐和酸盐度的影响.

主要方法:

猪被食的是NaCl或N-酸盐 (Na15).
在眼组织和血中测量了总和N标记的酸盐和酸盐的水平.
测量是在一次剂量和五次连续每日剂量Na15NO3后进行的.

主要成果:

一次Na¹⁵NO₃剂量在所有眼组织中显著增加¹⁵NO_{3-和¹⁵NO_{2-^-,特别是在水性和玻璃体中.}}
在摄入酸盐后,眼部组织显示¹⁵NO_{2^-的丰富度高于血.}
与单次剂量相比,每日多次服用Na¹⁵NO₃不会导致酸盐或酸盐的进一步积累.

结论:

饮食中酸盐补充剂会影响眼睛中的酸盐和酸盐水平.
这些发现支持饮食中酸盐/酸盐的潜在治疗用途,以调节眼睛组织中的NO水平.

关键词:

饮食中的酸盐氧化氧化的使用方法亚酸盐的化物猪的眼睛猪的眼睛

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2° Amines to N-Nitrosamines: Reaction with NaNO2

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Secondary amines react with nitrous acid to form N-nitrosamines, as depicted in Figure 1. Nitrous acid, a weak and unstable acid, is formed in situ from an aqueous solution of sodium nitrite and strong acids, such as hydrochloric acid or sulfuric acid, in cold conditions. In the presence of an acid, the nitrous acid gets protonated. The subsequent loss of water results in the formation of the electrophile known as nitrosonium ion.