短期饮食限制增强了与粘膜相关的抗炎循环不变T细胞反应
Brian Fazzone1, Erik M Anderson1, Jared M Rozowsky1
1Department of Surgery, Division of Vascular Surgery and Endovascular Therapy, University of Florida, Gainesville, FL 32611, USA.
Nutrients
|April 27, 2024
概括
短期饮食限制 (StDR) 可以通过改变肠道微生物和免疫细胞来减少炎症. 在粘膜关联不变T细胞 (MAITs) 中,StDR降低了促炎基因的调节,这表明它具有抗炎作用.
科学领域:
- 免疫学 免疫学 免疫学
- 微生物学 微生物学
- 营养科学 营养科学
背景情况:
- 短期蛋白质卡路里饮食限制 (StDR) 是一个潜在的手术前策略,以减少炎症.
- 人们还没有完全理解STDR,肠道微生物群和系统性免疫之间的相互作用.
- 粘膜关联不变T细胞 (MAITs) 链接先天性和适应性免疫,对肠道微生物的变化敏感.
研究的目的:
- 为了研究StDR对MAIT细胞转录组学的影响.
- 分析在StDR期间肠道微生物组成的变化.
- 探索STDR,肠道微生物群和MAIT细胞反应之间的关系.
主要方法:
- 从健康的志愿者中对外围血液单核细胞 (PBMCs) 的单细胞RNA测序,接受了4天的StDR.
- 排序便样本以评估肠道微生物物种的丰富程度.
- 在饮食干预之前,期间和之后收集血液和便样本.
主要成果:
- 在PBMC中,MAIT细胞占2.4%.
- 因此,StDR导致MAIT激活和促炎基因 (例如CD69,IL1,IL6,TNFα) 的下调,以及抗炎IL10信号基因的上调.
- 在StDR期间观察到MAIT刺激的 * 细菌体 * 种类的丰度下降.
结论:
- StDR可能会诱导一种抗炎MAIT细胞表型.
- 这种免疫类型的转变可能是由T细胞受体依赖信号的变化中介的.
- 在STDR期间肠道微生物组成的变化可能会导致观察到的抗炎性免疫反应.
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