3D人造皮肤平台用于调查与怀孕相关的皮肤颜色
Uiechan Jeong1, Sunhee Yoon2, Sungjin Park3
1Department of Mechanical Engineering, Inha University, 100 Inha-ro, Incheon 22212, Republic of Korea.
Micromachines
|April 27, 2024
概括
一个新的3D人造皮肤平台模仿孕妇皮肤来研究色素. 这个平台准确地模拟了怀孕期间的黑色素生产变化,与2D培养不同,提供了一个新的研究工具.
科学领域:
- 生物材料工程 生物材料工程
- 皮肤病学 皮肤病学
- 生殖生物学 生殖生物学
背景情况:
- 怀孕涉及显著的生理变化,影响皮肤色素.
- 现有的二维细胞培养模型不能准确地复制体内皮肤对与怀孕相关的荷尔蒙和环境变化的反应.
- 准确建模怀孕期间的色素变化对于开发有效治疗方法至关重要.
研究的目的:
- 开发和验证一个模拟孕妇皮肤颜色特征的3D人造皮肤平台.
- 将3D人造皮肤平台中的黑色素表达模式与在模拟妊娠条件下的传统2D细胞培养中的黑色素表达模式进行比较.
主要方法:
- 制造双层水凝 (I型原和纤维素) 用于3D人造皮肤结构.
- 3D人造皮肤平台的动态培养.
- 通过控制氧气度 (2.5-3%) 和17β-雌二醇 (E2) 水平 (17 ng/mL) 来模拟怀孕环境.
主要成果:
- 3D人造皮肤平台在模拟妊娠条件下 (低氧和高E2) 显示出黑色素表达显著增加2-6倍.
- 这些结果与孕妇体内观察到的黑色素表达趋势相关联.
- 两维细胞培养也未能在黑色素表达方面显示出类似的显著趋势.
结论:
- 开发的3D人造皮肤平台有效地复制了怀孕引起的色素变化.
- 该平台作为一个优越的体外模型与2D培养相比,用于研究怀孕期间的色素障碍.
- 该平台具有研究色素治疗和理解怀孕期间皮肤生理学的潜力.
相关概念视频
01:19Pigmentation
The color of the skin is influenced by a number of pigments, including melanin, carotene, and hemoglobin. Recall that melanin is produced by cells called melanocytes, which are found scattered throughout the stratum basale of the epidermis. The melanin is transferred to the keratinocytes via melanosomes.
Melanin occurs in two primary forms: eumelanin that provides black and brown pigment and pheomelanin that provides red color. Dark-skinned individuals produce more melanin than those with pale...
Melanin occurs in two primary forms: eumelanin that provides black and brown pigment and pheomelanin that provides red color. Dark-skinned individuals produce more melanin than those with pale...
01:14Changes in Skin Color: Clinical Perspectives
The first thing a clinician sees is the skin, so the examination of the skin should be part of any thorough physical examination. Most skin disorders are relatively benign, but a few, including melanomas, can be fatal if untreated. A couple of the more noticeable disorders, albinism and vitiligo, affect the appearance of the skin and its accessory organs.
Albinism
Albinism is a genetic disorder that affects (completely or partially) the coloring of skin, hair, and eyes. The defect is primarily...
Albinism
Albinism is a genetic disorder that affects (completely or partially) the coloring of skin, hair, and eyes. The defect is primarily...