作为骨肌肉缩的改善剂,Lycii Radicis皮质的潜力
Rak Ho Son1,2, Myeong Il Kim2, Hye Mi Kim1
1College of Pharmacy and Institute of Pharmaceutical Science and Technology, Hanyang University, 55 Hanyangdaehak-ro, Ansan 15588, Republic of Korea.
Pharmaceuticals (Basel, Switzerland)
|April 27, 2024
概括
莱西基根茎皮质提取物 (LRCE) 显示出对肌肉缩的保护作用. 在临床前模型中,LRCE改善了肌肉细胞的健康和功能,这表明它有可能用于治疗肌肉衰竭疾病.
科学领域:
- 药理学 药理学是指药理学的学科.
- 细胞生物学 细胞生物学
- 肌肉生理学 肌肉生理学
背景情况:
- Lycii Radicis Cortex (LRC) 是一种东亚传统药物,具有多种健康益处.
- LRC对骨肌肉缩的影响在很大程度上仍未被探索.
- 肌肉缩是一种具有重大健康影响的衰弱状况.
研究的目的:
- 研究Lycii Radicis Cortex提取物 (LRCE) 对德甲 (DEX) 诱导的骨肌缩的保护作用.
- 评估LRCE对肌肉缩背后的细胞机制的影响在体外和体内.
- 探索LRCE作为肌肉消耗疾病的潜在治疗剂.
主要方法:
- 利用C2C12神经管和小鼠模型研究DEX诱导的肌肉缩.
- 采用了免疫光染色,qPCR和西方斑点分析.
- 评估了氧化应激,亡,ATP水平,蛋白质合成/降解,以及肌肉组织形态.
主要成果:
- 在实验室中,LRCE改善了髓管直径,融合指数和线粒体含量.
- 在增加ATP水平的同时,LRCE减少了氧化应激 (ROS) 和亡.
- LRCE上调调节了肌原蛋白和肌重链 (MHC) 的表达,增强了蛋白质合成,降低了降解.
- 在体内,LRCE恢复了小牛的厚度,调节了关键缩相关基因表达 (MuRF1,atrogin-1,IGF-1),并修复了胃肌缩.
结论:
- 在细胞和动物模型中,LRCE证明了对DEX诱导的肌肉缩具有显著的保护作用.
- LRCE通过增强蛋白质合成,减少降解,减轻氧化应激,改善线粒体功能来起作用.
- 临床前发现表明,LRCE在预防和治疗肌肉缩方面具有前景.
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